Presence of disease resistance genes in tomato germplasm revealed by gene-based or gene-linked molecular markers.

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2025-03-21 eCollection Date: 2025-04-01 DOI:10.1007/s11032-025-01557-1

Luyao Yang, Jie Ren, Huanhuan Yang, Tao Zhou, Wencai Yang

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引用次数: 0

Abstract

Tomato (Solanum lycopersicum L.) is a worldwide economically important vegetable crop, but the production is severely threatened by diseases and pests. Clarifying the presence of disease resistant genes in tomato germplasm is helpful for the development and deployment of resistant cultivars. In this study, 21 genes for resistance to 10 diseases were examined in a collection of 401 tomato accessions using gene-associated molecular markers. The results showed that the presence of the 21 genes in the 401 tomato accessions ranged from 0.2% to 66.1%. Frequencies (27.9-66.1%) of I-1, I-2, Sm, Tm-2a, and Ph-3 genes were higher than other genes, while frequencies (0.2-1.2%) of Ty-2, Ty-4, Mi-HT, I-3, and Tm-2 genes were very low. Based on the marker genotypes, 86.3% accessions carried at least one of disease resistance genes. Most (84.2%) Contemporary Processing F₁ (CPF) carried 4-8 genes and 74.1% Contemporary Fresh-market F₁ (CFF) carried 4-6 genes, while 87.4% Contemporary Processing Inbreds (CPI) carried 1-5 genes and 85.3% Contemporary Fresh-market Inbreds (CFI) carried 1-4 genes. Furthermore, the numbers of disease resistance genes were lower in Latin American Landrace (1-3), Vintage (1-3), and CFI (1-4) than in CFF (1-8), CPI (1-7), and CPF (2-10). Among multiple markers used for detection of the single gene, markers CAUTy4 and 18IY13 had the highest consistency (100%) of genotypes for the Ty-4 gene, followed by SM-InDel and InDel-FT-29 (94.0%) for the Sm gene, while markers AW910upF2R3, 20IY10, and TG0302 for detecting the Ty-2 gene had the lowest consistency (44.4%). Disease evaluation confirmed the accuracy of marker-assisted identification of corresponding genes except that the accessions carrying the Ty-1 gene uniquely detected by the CAPS1 marker were susceptible to tomato yellow leaf curl virus. The data obtained here provide valuable information for marker-assisted selection of these disease resistance genes and the use of these germplasm in tomato breeding and production.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01557-1.

查看原文本刊更多论文

利用基因标记或基因连锁分子标记揭示番茄种质中抗病基因的存在。

番茄（Solanum lycopersicum L.）是一种世界性的重要经济蔬菜作物，但其生产受到病虫害的严重威胁。明确番茄种质资源中抗病基因的存在，有助于抗病品种的开发和配置。本研究利用基因相关分子标记对401份番茄材料中21个抗病基因进行了检测。结果表明，21个基因在401份番茄材料中的存在率在0.2% ~ 66.1%之间。I-1、I-2、Sm、Tm-2a和Ph-3基因频率较高（27.9 ~ 66.1%），而Ty-2、Ty-4、Mi-HT、I-3和Tm-2基因频率极低（0.2 ~ 1.2%）。根据标记基因型，86.3%的材料携带至少一种抗病基因。大多数（84.2%）当代加工F1 （CPF）携带4-8个基因，74.1%的当代生鲜F1 （CFF）携带4-6个基因，87.4%的当代加工自交系（CPI）携带1-5个基因，85.3%的当代生鲜自交系（CFI）携带1-4个基因。此外，拉丁美洲长白（1-3）、古着（1-3）和CFI（1-4）的抗病基因数量低于CFF（1-8）、CPI（1-7）和CPF（2-10）。在检测Ty-4基因的多个标记中，CAUTy4和18IY13标记对Ty-4基因型的一致性最高（100%），Sm基因的一致性其次是Sm - indel和InDel-FT-29(94.0%)，而检测Ty-2基因的AW910upF2R3、20IY10和TG0302标记的一致性最低（44.4%）。病害评价证实了相应基因的标记辅助鉴定的准确性，但携带CAPS1标记唯一检测到的Ty-1基因的材料对番茄黄卷叶病毒易感。本研究为番茄抗病基因的标记辅助选择和种质资源在番茄育种和生产中的应用提供了有价值的信息。补充资料：在线版本包含补充资料，下载地址：10.1007/s11032-025-01557-1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.