Enhanced expression of miR-128 in peripheral blood mononuclear cells: a potent candidate diagnostic biomarker for multiple sclerosis.

IF 1.7 4区医学 Q3 CLINICAL NEUROLOGY

Neurological Research Pub Date : 2025-03-23 DOI:10.1080/01616412.2025.2481443

Hossein Akhgari, Alireza Ahmadi, Abedeh Rezaei, Ali Mohammadi, Reza Safaralizadeh

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引用次数: 0

Abstract

Objectives: Millions of people worldwide, especially young adults, are affected by multiple sclerosis (MS), a demyelinating and autoimmune condition of the central nervous system (CNS). Many studies have focused on the diagnostic value of miRNAs circulating in body fluids of MS patients. Thus, this study aimed to assess the expression of miR-128 and its potential as a biomarker for MS in the Iranian population.

Methods: This study recruited 85 MS patients and 30 healthy matched controls. Following the collection of blood samples, serum was isolated. RNA was then extracted, cDNA was synthesized, and miR-128 expression was measured by real-time PCR.

Results: There was significant overexpression of miR-128 in MS patients in comparison with healthy controls (p-value <0.0001). In addition, there was a rather notable association between the subtypes of MS samples and the expression levels of miR-128. ROC analysis showed an AUC of 0.9111 (95%CI 0.85-0.97, p-value <0.0001) with sensitivity and specificity of 80.00% and 93.33%, respectively.

Conclusion: The expression of miR-128 was significantly higher in PBMCs of MS patients in comparison with healthy controls. Additionally, miR-128 could be an excellent biomarker for MS patient diagnosis.

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外周血单核细胞中 miR-128 的表达增强：多发性硬化症的有效候选诊断生物标记物。

目标：多发性硬化症（MS）是中枢神经系统（CNS）的一种脱髓鞘和自身免疫性疾病，全世界有数百万人，尤其是年轻的成年人深受其害。许多研究都关注多发性硬化症患者体液中循环的 miRNAs 的诊断价值。因此，本研究旨在评估伊朗人群中 miR-128 的表达及其作为多发性硬化症生物标志物的潜力：方法：本研究招募了 85 名多发性硬化症患者和 30 名健康匹配对照组。收集血液样本后，分离血清。然后提取 RNA，合成 cDNA，并通过实时 PCR 检测 miR-128 的表达：结果：与健康对照组相比，多发性硬化症患者体内的 miR-128 明显过表达（p 值结论：miR-128 在多发性硬化症患者体内的表达明显高于健康对照组：与健康对照组相比，多发性硬化症患者的PBMCs中miR-128的表达明显升高。此外，miR-128 可能是诊断多发性硬化症患者的极佳生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurological Research 医学-临床神经学

CiteScore

3.60

自引率

0.00%

发文量

116

审稿时长

5.3 months

期刊介绍： Neurological Research is an international, peer-reviewed journal for reporting both basic and clinical research in the fields of neurosurgery, neurology, neuroengineering and neurosciences. It provides a medium for those who recognize the wider implications of their work and who wish to be informed of the relevant experience of others in related and more distant fields. The scope of the journal includes: •Stem cell applications •Molecular neuroscience •Neuropharmacology •Neuroradiology •Neurochemistry •Biomathematical models •Endovascular neurosurgery •Innovation in neurosurgery.