Zhike Erfang Alleviates MRSA-Induced Pneumonia by Inhibiting TRAF6 and Activating NLRP3 Inflammatory Body.

IF 4.2 2区医学 Q2 IMMUNOLOGY

Journal of Inflammation Research Pub Date : 2025-03-17 eCollection Date: 2025-01-01 DOI:10.2147/JIR.S466737

Lian-Qing Zhang, Wen-Can Zheng, Wen-Yan Li

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Abstract

Purpose: The therapeutic effects of Zhike Erfang in modulating the cellular responses and immune microenvironment associated with MRSA-induced acute lung injury remain unclear. This study aims to elucidate the potential mechanisms by which Zhike Erfang mitigate the cellular and molecular effects of MRSA in a laboratory model.

Patients and methods: A mouse model of acute lung injury was established using heat-inactivated MRSA. Lung tissue and bronchoalveolar lavage fluid were collected for analysis. Macrophages were pretreated with Zhike Erfang for 30 minutes before exposure to heat-inactivated MRSA for 24 hours. Protein expressions of TRAF6, iNOS, TNF-α, IL-1β, NLRP3, and caspase-1 in lung tissues were quantified using Western blot. The content of LDH was detected by the lactate dehydrogenase cytotoxicity test kit.

Results: Zhike Erfang significantly reduced the expression of iNOS, LDH, TNF-α, IL-1β, NLRP3, and caspase-1 in a dose-dependent manner in lung tissues from the MRSA model. Zhike Erfang inhibited the expression of TRAF6.

Conclusion: Zhike Erfang can alleviate pneumonia caused by MRSA by inhibiting TRAF6 and inducing NLRP3 inflammatory body activation.

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止咳方通过抑制TRAF6和激活NLRP3炎性体缓解mrsa诱导的肺炎。

目的：止咳二方对mrsa诱导的急性肺损伤细胞反应和免疫微环境的调节作用尚不清楚。本研究旨在通过实验室模型阐明止咳二方减轻MRSA细胞和分子效应的潜在机制。患者和方法：采用热灭活MRSA建立小鼠急性肺损伤模型。采集肺组织及支气管肺泡灌洗液进行分析。巨噬细胞用致科二方预处理30分钟后，热灭活MRSA处理24小时。Western blot检测肺组织中TRAF6、iNOS、TNF-α、IL-1β、NLRP3、caspase-1蛋白的表达。乳酸脱氢酶细胞毒性检测试剂盒检测乳酸脱氢酶含量。结果：栀科二方显著降低MRSA模型肺组织中iNOS、LDH、TNF-α、IL-1β、NLRP3、caspase-1的表达，且呈剂量依赖性。止咳二方抑制TRAF6的表达。结论：止咳二方可通过抑制TRAF6、诱导NLRP3炎性体激活来缓解MRSA所致肺炎。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Inflammation Research Immunology and Microbiology-Immunology

CiteScore

6.10

自引率

2.20%

发文量

658

审稿时长

16 weeks

期刊介绍： An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.