CadmiLume: A Novel Smartphone-Based Bioluminescence Color-Tuning Assay and Biosensor for Cadmium and Heavy Metal Detection in Water Samples.

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
Vadim R Viviani, Murilo S Teixeira, Gabriel F Pelentir
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引用次数: 0

Abstract

Heavy metal contamination of soil and water is a growing environmental concern, especially mercury, lead, and cadmium. Therefore, fast and reliable methodologies to assess contamination in the field are in demand. However, many methodologies require laborious, expensive, and cumbersome equipment that is not convenient for rapid field analysis. Mobile phone technology coupled with bioluminescent assays provides accessible hands-on alternatives that has already been shown to be feasible. Previously, we demonstrated that firefly luciferases can be harnessed as luminescence color-tuning sensors for toxic metals. An assay based on such a principle was already successfully applied for teaching biochemistry laboratory lessons, which demonstrates the effect of cadmium on enzyme function based on bioluminescence color change. For analytical detection of cadmium in water, here, we developed a novel bioluminescence assay using the cadmium-sensitive Amydetes vivianii firefly luciferase coupled with a cell phone provided with a program to quantify cadmium concentration based on luminescence color discrimination. The application has proven to be efficient with high precision between 0.10 and 2 mM of cadmium, being appliable to diluted water samples (0.1-2 µM) upon concentration and relying on reference cadmium standards values. The light emitted by the reference standards and samples in a dark box is captured by the smartphone's camera, which, using computer vision, automatically quantifies cadmium according to the RGB color. CadmiLume is a simple and easy luminescent enzymatic biosensor for cadmium contamination in water samples, which instantaneously can provide results with the convenience of a smartphone in the palm of one's hands.

CadmiLume:一种基于智能手机的新型生物发光调色试验和生物传感器,用于水样中镉和重金属的检测。
土壤和水的重金属污染是一个日益严重的环境问题,尤其是汞、铅和镉。因此,需要快速可靠的方法来评估现场的污染。然而,许多方法需要费力、昂贵和笨重的设备,不便于快速现场分析。移动电话技术与生物发光测定相结合,提供了可操作的替代方法,这种方法已被证明是可行的。在此之前,我们证明了萤火虫荧光素酶可以作为有毒金属的发光颜色调节传感器。基于这一原理的实验已成功应用于生物化学实验课的教学中,通过生物发光的颜色变化证明了镉对酶功能的影响。为了分析水中镉的检测,我们开发了一种新的生物发光法,利用镉敏感的amydees vivianii萤火虫荧光素酶与提供了基于发光颜色识别的程序的手机相结合,来定量镉浓度。该应用程序已被证明是高效的,在0.10和2mm的镉之间具有高精度,适用于浓度(0.1-2µM)的稀释水样,并依赖于参考镉标准值。参考标准和样品在暗箱中发出的光被智能手机的摄像头捕获,该摄像头使用计算机视觉,根据RGB颜色自动定量镉。CadmiLume是一种简单易用的发光酶生物传感器,用于检测水样中的镉污染,它可以像在手掌上的智能手机一样立即提供结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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