Is the endophyte-based plant protection against aphids mediated by changes in the insect microbiome?

IF 2.9 1区 农林科学 Q1 ENTOMOLOGY
Daniel A Bastías, Luis Carvalho, Ruy Jáuregui, Richard D Johnson, Wei Zhang, Pedro E Gundel
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Abstract

Aphids are important herbivores in natural and managed environments. We studied the response of aphids and their associated microbiota to the presence of the fungal endophyte Epichloë sp. LpTG-3 strain AR37, and the AR37-derived alkaloids in plants. We hypothesized that AR37 and/or AR37-derived alkaloids would reduce the aphid performance, and that this reduction would be associated with endophyte-mediated changes in the abundance, composition, and diversity of beneficial bacterial endosymbionts of aphids (e.g., Buchnera). Plants of Lolium perenne associated with AR37 variants able (wild type and ∆idtA) and unable (∆idtM) to produce indole diterpene alkaloids were challenged with Rhopalosiphum padi aphids. We measured aphid population size, plant biomass, and the abundance, composition and diversity of the aphid's bacterial microbiota. The presence of AR37 increased the resistance of plants against R. padi aphids via the production of indole diterpene alkaloids, and this effect was independent of the plant biomass. The endophyte-mediated reduction in aphid performance was not associated with changes in the abundance, composition and diversity of the insect's bacterial microbiota. However, we cannot rule out that the reduction in aphid performance could be associated with a putative endophyte effect on the bacterial provision of benefits to aphids. Our study highlighted the protective role of endophyte-derived indole diterpene alkaloids against aphids. Further investigations will be needed to determine if there is a link between the endophyte-mediated aphid resistance and the integrity of the insect's bacterial microbiota.

蚜虫是自然环境和管理环境中的重要食草动物。我们研究了蚜虫及其相关微生物群对植物中真菌内生菌 Epichloë sp. LpTG-3 菌株 AR37 和 AR37 衍生生物碱的反应。我们假设 AR37 和/或 AR37 衍生生物碱会降低蚜虫的表现,而这种降低与内生真菌介导的蚜虫有益细菌内共生体(如 Buchnera)的丰度、组成和多样性的变化有关。用能产生吲哚二萜生物碱的 AR37 变体(野生型和 ∆idtA)和不能产生吲哚二萜生物碱的 AR37 变体(∆idtM)与蚜虫 Rhopalosiphum padi 进行挑战。我们测量了蚜虫种群数量、植物生物量以及蚜虫细菌微生物群的丰度、组成和多样性。通过产生吲哚二萜生物碱,AR37的存在增强了植物对R. padi蚜虫的抵抗力,而且这种影响与植物生物量无关。内生菌介导的蚜虫表现下降与昆虫细菌微生物群的丰度、组成和多样性变化无关。但是,我们不能排除蚜虫表现的下降可能与细菌为蚜虫提供益处的假定内生菌效应有关。我们的研究强调了内生菌衍生的吲哚二萜生物碱对蚜虫的保护作用。要确定内生菌介导的蚜虫抗性与昆虫细菌微生物群的完整性之间是否存在联系,还需要进一步的研究。
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来源期刊
Insect Science
Insect Science 生物-昆虫学
CiteScore
7.80
自引率
5.00%
发文量
1379
审稿时长
6.0 months
期刊介绍: Insect Science is an English-language journal, which publishes original research articles dealing with all fields of research in into insects and other terrestrial arthropods. Papers in any of the following fields will be considered: ecology, behavior, biogeography, physiology, biochemistry, sociobiology, phylogeny, pest management, and exotic incursions. The emphasis of the journal is on the adaptation and evolutionary biology of insects from the molecular to the ecosystem level. Reviews, mini reviews and letters to the editor, book reviews, and information about academic activities of the society are also published.
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