Effects of supplemental 25-hydroxyvitamin D3 on growth performance, physiological responses, and gene expression of skeletal muscle growth of finishing beef cattle.

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Tainá E Martins, Vinícius N Gouvêa, Alexandre Perdigão, Maria Betania Niehues, Cyntia L Martins, Danilo D Millen, Tiago S Acedo, Victor V Carvalho, Luis F M Tamassia, Mario D B Arrigoni
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Abstract

The objective of this experiment was to evaluate the effects of 25-hydroxyvitamin D3 (25(OH)D3) supplementation on growth performance, carcass characteristics, meat quality, and the expression of genes related to anabolism of skeletal muscle in finishing beef cattle. One hundred and twenty Nellore bulls (initial body weight (BW) = 376 ± 20 kg) were blocked by initial BW, allocated to 24 pens (5 bulls/pen) and pens were assigned to one of three treatments during a 96 days feeding experiment: control: high concentrate basal diet (11% roughage; NEg = 1.16 Mcal/kg dry matter) with no supplemental 25(OH)D3 (0 mg of 25(OH)D3; n = 8 pens); basal diet containing supplemental 25(OH)D3 to provide 1 mg/animal/day (1 mg of 25(OH)D3; n = 8 pens), 3) basal diet containing supplemental 25(OH)D3 to provide 3 mg/animal/day (3 mg of 25(OH)D3; n = 8 pens). The dietary supplementation of 25(OH)D3 did not affect final BW, dry matter intake, average daily gain, and feed efficiency (P ≥ 0.32). Dressing percentage increased quadratically (P = 0.03) and Longissimus muscle area tended to increase quadratically (P = 0.09) with increasing levels of 25(OH)D3 supplementation. A treatment × day interaction was observed for plasma concentration of 25(OH)D3 (P < 0.001). No difference in plasma 25(OH)D3 concentration between treatments was observed at the beginning of the experiment (P > 0.05), but on days 37 and 95, plasma 25(OH)D3 was greater (P ≤ 0.05) for bulls fed 3 mg, followed by 1 mg, and 0 mg of 25(OH)D3. No effects of dietary supplementation of 25(OH)D3 were observed on meat quality attributes (P ≥ 0.24), except for meat pH that linearly increased (P < 0.01). The percentage of fat in the carcasses decreased linearly (P = 0.03) with increasing levels of 25(OH)D3 supplementation, followed by a numerical increase (P = 0.11) in the percentage of muscle. The gene expression of insulin-like growth factor-2 (IGF2), mammalian target of rapamycin, and myostatin tended (P ≤ 0.10), and IGF1 increased linearly (P = 0.04) with increasing levels of 25(OH)D3. In summary, the inclusion of 25-hydroxyvitamin D3 in feedlot diets may go beyond regulating calcium metabolism and meat quality only. Dietary supplementation of 1 mg of 25(OH)D3 for finishing beef cattle increased carcass dressing percentage and Longissimus muscle area by the upregulation of genes associated with skeletal muscle growth insulin-like growth factor-1 and 2, mammalian target of rapamycin, and myostatin.

添加25-羟基维生素D3对肥育肉牛生长性能、生理反应及骨骼肌生长基因表达的影响
本试验旨在探讨饲粮中添加25(OH)D3对育肥肉牛生长性能、胴体特性、肉品质及骨骼肌合成代谢相关基因表达的影响。选取初始体重= 376±20 kg的Nellore公牛120头,分配到24个栏中(5头/栏),每栏分为3个处理:对照组:高精料基础饲粮(11%粗饲料;NEg = 1.16 Mcal/kg干物质),不添加25(OH)D3 (0 mg 25(OH)D3);N = 8支笔);在基础饲粮中添加25(OH)D3,提供1 mg/ d (1 mg 25(OH)D3);n = 8头),3)基础饲粮中添加25(OH)D3,提供3 mg/只/天(3 mg 25(OH)D3;N = 8支笔)。饲粮中添加25(OH)D3对最终体重、干物质采食量、平均日增重和饲料效率无显著影响(P≥0.32)。随着25(OH)D3添加水平的增加,屠宰率呈二次曲线上升(P = 0.03),最长肌面积呈二次曲线上升(P = 0.09)。血浆25(OH)D3浓度与治疗期存在交互作用(P < 0.001)。试验开始时,各处理公牛血浆25(OH)D3浓度无显著差异(P < 0.05),但在第37天和第95天,25(OH)D3添加量为3 mg、1 mg和0 mg的公牛血浆25(OH)D3浓度较高(P≤0.05)。饲粮中添加25(OH)D3对肉质性状无影响(P≥0.24),但肉质pH呈线性升高(P < 0.01)。随着25(OH)D3添加水平的增加,胴体脂肪百分比呈线性下降(P = 0.03),肌肉百分比呈数值上升(P = 0.11)。随着25(OH)D3水平的升高,胰岛素样生长因子-2 (IGF2)、哺乳动物雷帕霉素靶蛋白和肌肉生长抑制素的基因表达趋于平缓(P≤0.10),IGF1呈线性升高(P = 0.04)。综上所述,在饲粮中添加25-羟基维生素D3可能不仅仅是调节钙代谢和肉品质。肉牛育肥期饲粮中添加1 mg 25(OH)D3通过上调骨骼肌生长胰岛素样生长因子-1和2、哺乳动物雷帕霉素靶蛋白和肌肉生长抑制素相关基因,提高了胴体屠宰率和最长肌面积。
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来源期刊
Journal of animal science
Journal of animal science 农林科学-奶制品与动物科学
CiteScore
4.80
自引率
12.10%
发文量
1589
审稿时长
3 months
期刊介绍: The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year. Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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