Elicitor-driven enhancement of phenolic compounds in geranium callus cultures: phytochemical profiling via LC-MS/MS and biological activities.

IF 3.8 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Frontiers in Chemistry Pub Date : 2025-03-07 eCollection Date: 2025-01-01 DOI:10.3389/fchem.2025.1537877

Amine Elbouzidi, Mohamed Taibi, Abdellah Baraich, Mounir Haddou, Ramzi A Mothana, Sami A Alsufyani, Hany W Darwish, Roland Molinié, Jean-Xavier Fontaine, Ophélie Fliniaux, François Mesnard, Mohamed Addi

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引用次数: 0

Abstract

This research explores the effects of chitosan (CHT) and salicylic acid (SA) as elicitors on the production of phenolic and flavonoid compounds in Pelargonium graveolens Hort. Callus cultures on solid media, aiming to enhance antioxidant, anti-tyrosinase, and anti-elastase properties. Calli were treated with various concentrations of CHT (25, 50, 75, and 100 mg/mL) and SA (25, 50, 75, and 100 µM), and their phytochemical profiles were examined through LC-MS/MS analysis. The findings indicated that salicylic acid (SA) and chitosan (CHT) notably enhanced the levels of total phenolic content (TPC) and total flavonoid content (TFC). The greatest increase in TPC was seen in cultures treated with 25 µM of salicylic acid (SA₂), recording 336.80 ± 8.35 mg/100 g dry weight (DW), and in cultures treated with 100 mg/mL of chitosan (CHT₅), which showed 325.74 ± 7.81 mg/100 g DW. Among individual phenolics, kaempferol showed a remarkable increase under SA₂ (192.82 ± 17.99 mg/100 g DW) compared to the control (103.68 ± 5.00 mg/100 g DW), and CHT₅ treatment (119.68 ± 12.01 mg/100 g DW). Additionally, rutin accumulation peaked at 30.64 ± 3.00 mg/100 g DW under SA₂ treatment. Antioxidant activities, measured by DPPH and TAC assays, were also enhanced, with SA₂ and CHT₅ treatments showing significant improvement over the control. The SA₂-elicited cultures exhibited superior anti-tyrosinase and anti-elastase activities, with IC₅₀ values of 51.43 ± 1.31 μg/mL, 35.42 ± 4.42 μg/mL, and 31.84 ± 0.60 μg/mL, respectively. These findings suggest that elicitors effectively boost the bioactive compound production in P. graveolens calli, and subsequently the biological activity, highlighting their potential in developing natural skincare products with antioxidant and anti-aging benefits.

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来源期刊

Frontiers in Chemistry Chemistry-General Chemistry

CiteScore

8.50

自引率

3.60%

发文量

1540

审稿时长

12 weeks

期刊介绍： Frontiers in Chemistry is a high visiblity and quality journal, publishing rigorously peer-reviewed research across the chemical sciences. Field Chief Editor Steve Suib at the University of Connecticut is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to academics, industry leaders and the public worldwide. Chemistry is a branch of science that is linked to all other main fields of research. The omnipresence of Chemistry is apparent in our everyday lives from the electronic devices that we all use to communicate, to foods we eat, to our health and well-being, to the different forms of energy that we use. While there are many subtopics and specialties of Chemistry, the fundamental link in all these areas is how atoms, ions, and molecules come together and come apart in what some have come to call the “dance of life”. All specialty sections of Frontiers in Chemistry are open-access with the goal of publishing outstanding research publications, review articles, commentaries, and ideas about various aspects of Chemistry. The past forms of publication often have specific subdisciplines, most commonly of analytical, inorganic, organic and physical chemistries, but these days those lines and boxes are quite blurry and the silos of those disciplines appear to be eroding. Chemistry is important to both fundamental and applied areas of research and manufacturing, and indeed the outlines of academic versus industrial research are also often artificial. Collaborative research across all specialty areas of Chemistry is highly encouraged and supported as we move forward. These are exciting times and the field of Chemistry is an important and significant contributor to our collective knowledge.