{"title":"Accelerating Bone Healing With METTL3 Overexpressed Adipose-Derived Stem Cells in Osteoporotic Rats.","authors":"Hui Tang, Zhenzhen Chen, Lu Zeng, Yuping Xie, Daowen Luo, Shuanglin Peng, Fangzhi Lou, Tianli Wu, Jingang Xiao","doi":"10.1111/cpr.70029","DOIUrl":null,"url":null,"abstract":"<p><p>The treatment of postmenopausal osteoporosis (OP) presents a multifaceted challenge. Nonetheless, emerging research indicates a significant association between the N6-methyladenosine (m6A) methylase METTL3 and osteogenesis in OP. To investigate Mettl3's impact on osteogenic potential and the underlying molecular mechanisms, an OP rat model was established via ovariectomy (OVX). Osteoporotic adipose-derived stem cells (OP-ASCs) were then isolated. Results indicated a significant downregulation of Mettl3 expression in OP-ASCs. Subsequently, OP-ASCs were transfected with overexpressed Mettl3 lentivirus and treated for Dickkopf-related protein-1 (DKK1). Overexpression of the Mettl3 gene led to increased levels of osteogenic factors. DKK1 attenuated osteoblastic differentiation capacity in the Mettl3 overexpression group by inhibiting the Wnt signalling pathway. Consistent results were observed in vivo experiments. In conclusion, overexpression of Mettl3 promotes osteogenesis in OP-ASCs by activating the Wnt/β-catenin pathway.</p>","PeriodicalId":9760,"journal":{"name":"Cell Proliferation","volume":" ","pages":"e70029"},"PeriodicalIF":5.9000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Proliferation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1111/cpr.70029","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
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Abstract
The treatment of postmenopausal osteoporosis (OP) presents a multifaceted challenge. Nonetheless, emerging research indicates a significant association between the N6-methyladenosine (m6A) methylase METTL3 and osteogenesis in OP. To investigate Mettl3's impact on osteogenic potential and the underlying molecular mechanisms, an OP rat model was established via ovariectomy (OVX). Osteoporotic adipose-derived stem cells (OP-ASCs) were then isolated. Results indicated a significant downregulation of Mettl3 expression in OP-ASCs. Subsequently, OP-ASCs were transfected with overexpressed Mettl3 lentivirus and treated for Dickkopf-related protein-1 (DKK1). Overexpression of the Mettl3 gene led to increased levels of osteogenic factors. DKK1 attenuated osteoblastic differentiation capacity in the Mettl3 overexpression group by inhibiting the Wnt signalling pathway. Consistent results were observed in vivo experiments. In conclusion, overexpression of Mettl3 promotes osteogenesis in OP-ASCs by activating the Wnt/β-catenin pathway.
期刊介绍：
Cell Proliferation
Focus:
Devoted to studies into all aspects of cell proliferation and differentiation.
Covers normal and abnormal states.
Explores control systems and mechanisms at various levels: inter- and intracellular, molecular, and genetic.
Investigates modification by and interactions with chemical and physical agents.
Includes mathematical modeling and the development of new techniques.
Publication Content:
Original research papers
Invited review articles
Book reviews
Letters commenting on previously published papers and/or topics of general interest
By organizing the information in this manner, readers can quickly grasp the scope, focus, and publication content of Cell Proliferation.
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