Functional genomics dissection of the nodulation autoregulation pathway (AON) in soybean (Glycine max)

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Peter M. Gresshoff, Chao Su, Huanan Su, April Hastwell, Yanyan Cha, Mengbai Zhang, Estelle B. Grundy, Xitong Chu, Brett J. Ferguson, Xia Li
{"title":"Functional genomics dissection of the nodulation autoregulation pathway (AON) in soybean (Glycine max)","authors":"Peter M. Gresshoff,&nbsp;Chao Su,&nbsp;Huanan Su,&nbsp;April Hastwell,&nbsp;Yanyan Cha,&nbsp;Mengbai Zhang,&nbsp;Estelle B. Grundy,&nbsp;Xitong Chu,&nbsp;Brett J. Ferguson,&nbsp;Xia Li","doi":"10.1111/jipb.13898","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The combination of mutation-based genetics and functional genomics has allowed a detailed dissection of the nodulation-induction and autoregulation of nodulation (AON) pathways of soybean. Applicable to all legumes, nodulation is induced by <i>Rhizobium</i>/<i>Bradyrhizobium-</i>produced lipopolysaccharides (Nod factors), perceived by Nod factor receptors (NFR1/NFR5 dimers), leading to cortical and pericycle cell divisions. These induce the production of CLAVATA3-like (CLE) peptides, which travel in the xylem to the shoot, where they are perceived by a receptor complex including a leucine-rich repeat (LRR) receptor kinase, encoded by <i>GmNARK</i>, <i>LjHAR1</i>, <i>MtSUNN</i> and closely related receptors in other legumes like <i>Phaseolus vulgaris</i> (common bean), <i>Pisum sativum</i> (pea), and <i>Glycine soja</i>. The activated receptor complex negatively regulates by phosphorylation of the constitutive synthesis of miR2111 in the shoot. This is normally is translocated via the phloem to the entire plant body, initiating suppression of a root-expressed Kelch repeat-containing F-box protein “Too Much Love (TML),” which in turn suppresses the nodule initiation cascade. Nodulation is therefore permitted during a developmental window between the induction and progress of the nodulation/cell division/infection cascade during the first few days after inoculation and the functional “readiness” of the AON cascade, delayed by the root–shoot–root loop. Loss-of-function mutations in <i>GmNARK</i> and <i>LjTML</i> result in excessive nodulation (supernodulation/hypernodulation/supernummary nodulation) as well as localized tolerance to externally applied nitrate. Recent analyses have indicated an interaction of the AON with lateral root formation as well as with the autoregulation of mycorrhization (AOM). Further details of the parallel functions of key points in this regulatory loop remain to be elucidated.</p></div>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"67 3","pages":"762-772"},"PeriodicalIF":9.3000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Integrative Plant Biology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jipb.13898","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

The combination of mutation-based genetics and functional genomics has allowed a detailed dissection of the nodulation-induction and autoregulation of nodulation (AON) pathways of soybean. Applicable to all legumes, nodulation is induced by Rhizobium/Bradyrhizobium-produced lipopolysaccharides (Nod factors), perceived by Nod factor receptors (NFR1/NFR5 dimers), leading to cortical and pericycle cell divisions. These induce the production of CLAVATA3-like (CLE) peptides, which travel in the xylem to the shoot, where they are perceived by a receptor complex including a leucine-rich repeat (LRR) receptor kinase, encoded by GmNARK, LjHAR1, MtSUNN and closely related receptors in other legumes like Phaseolus vulgaris (common bean), Pisum sativum (pea), and Glycine soja. The activated receptor complex negatively regulates by phosphorylation of the constitutive synthesis of miR2111 in the shoot. This is normally is translocated via the phloem to the entire plant body, initiating suppression of a root-expressed Kelch repeat-containing F-box protein “Too Much Love (TML),” which in turn suppresses the nodule initiation cascade. Nodulation is therefore permitted during a developmental window between the induction and progress of the nodulation/cell division/infection cascade during the first few days after inoculation and the functional “readiness” of the AON cascade, delayed by the root–shoot–root loop. Loss-of-function mutations in GmNARK and LjTML result in excessive nodulation (supernodulation/hypernodulation/supernummary nodulation) as well as localized tolerance to externally applied nitrate. Recent analyses have indicated an interaction of the AON with lateral root formation as well as with the autoregulation of mycorrhization (AOM). Further details of the parallel functions of key points in this regulatory loop remain to be elucidated.

Abstract Image

大豆(Glycine max)结瘤自调节通路(AON)的功能基因组学分析。
基于突变的遗传学和功能基因组学的结合,使得对大豆结瘤诱导和自动调节(AON)途径的详细剖析成为可能。适用于所有豆科植物,根瘤菌/缓生根瘤菌产生的脂多糖(结瘤因子)诱导结瘤,由结瘤因子受体(NFR1/NFR5二聚体)感知,导致皮质和中柱鞘细胞分裂。这些诱导clavata3样(CLE)肽的产生,这些肽在木质部传播到茎部,在那里它们被包括富含亮氨酸重复(LRR)受体激酶的受体复合物感知,该受体激酶由gmark、LjHAR1、MtSUNN和其他豆类(如菜豆)、豌豆(Pisum sativum)和甘氨酸大豆等密切相关的受体编码。激活的受体复合体通过磷酸化组成miR2111在茎部的合成负调控。这通常是通过韧皮部转移到整个植物体,开始抑制根表达的含有Kelch重复的F-box蛋白“太多的爱(TML)”,这反过来又抑制了根瘤形成级联。因此,在接种后的最初几天内,在结瘤/细胞分裂/感染级联的诱导和进展与AON级联的功能“准备”之间的发育窗口期间,结瘤是允许的,这被根-枝-根循环延迟。GmNARK和LjTML的功能缺失突变导致过度结瘤(超结/超结/超结)以及对外用硝酸盐的局部耐受。最近的分析表明,AON与侧根形成以及菌根化的自动调节(AOM)相互作用。这一调控回路中关键点的平行功能的进一步细节仍有待阐明。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信