Interaction between Bacteroides and HG-type pectins with different molecular weights

IF 7.7 1区 化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Song Li , Xiaomao Peng , Zengbo Wang , Chunhua Chen , Xin Li , Qixing Nie , Xiaojun Huang , Shuigen Bian , Junyi Yin , Steve W. Cui , Huizi Tan , Shaoping Nie
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引用次数: 0

Abstract

Pectins, a complex class of polysaccharides, are prominently represented by HG-chains, which are both abundant and extensively studied. Bacteroides species exhibit a remarkable ability to metabolize plant- and animal-derived polysaccharides, including the degradation of HG-type pectins through polysaccharide utilization locus (PUL). However, the effects of structurally diverse HG-type pectins on PUL expression, metabolite production, and metabolic pathways of Bacteroides remain unclear. Addressing this, this study chose HG-type pectins with different molecular weights from citrus (CP) and pomelo (PP), identified Bacteroides species capable of utilizing these pectins, studied the resulting metabolites through non-targeted metabolomics coupled with short-chain fatty acids analysis, and examined the activation of PUL and metabolic pathways by transcriptomic studies. The results showed that Bacteroides thetaiotaomicron A4 and Bacteroides caccae K9 could utilize HG-type pectins with different molecular weights. The production of propionic acid by Bacteroides thetaiotaomicron A4 was significantly affected by the molecular weight of pectins. Utilizing CP, B. thetaiotaomicron A4 enriched metabolites such as carbohydrates, amino acids, peptides, amines, and significantly enhanced pathways such as sphingolipid metabolism and drug metabolism (other enzymes) through PUL75, PUL63, and PUL55. In contrast, when using PP, B. thetaiotaomicron A4 enriched similar metabolites and further upregulated pathways related to sphingolipid metabolism and pyrimidine metabolism. The molecular weight of HG-type pectins differentially affected the expression of carbohydrate-active enzymes and metabolic pathways, resulting in different metabolite profiles. This study aims to contribute to the understanding of structure-activity relationship between pectins and gut microbiota and to inform precision nutrition strategies.

Abstract Image

拟杆菌与不同分子量hg型果胶的相互作用。
果胶是一类复杂的多糖,以hg链为代表,其含量丰富,研究广泛。拟杆菌类具有显著的代谢植物和动物来源的多糖的能力,包括通过多糖利用位点(PUL)降解hg型果胶。然而,结构不同的hg型果胶对拟杆菌的PUL表达、代谢物产生和代谢途径的影响尚不清楚。为此,本研究从柑橘(CP)和柚子(PP)中选择了不同分子量的hg型果胶,鉴定了能够利用这些果胶的拟杆菌(Bacteroides)物种,并通过非靶向代谢组学结合短链脂肪酸分析研究了产生的代谢产物,并通过转录组学研究了PUL的激活和代谢途径。结果表明,拟杆菌(Bacteroides thetaiotaomicron A4)和拟杆菌(Bacteroides caccae K9)均能利用不同分子量的hg型果胶。果胶分子量对拟杆菌(Bacteroides thetaiotaomicron A4)产丙酸有显著影响。利用CP, B. thetaiotaomicron A4富集了碳水化合物、氨基酸、多肽、胺等代谢物,并通过PUL75、PUL63和PUL55显著增强了鞘脂代谢和药物代谢(其他酶)等途径。相比之下,当使用PP时,B. thetaiotaomicron A4富集了类似的代谢物,并进一步上调了鞘脂代谢和嘧啶代谢相关的途径。hg型果胶分子量不同,影响糖活性酶的表达和代谢途径,导致不同的代谢产物谱。本研究旨在为了解果胶与肠道菌群之间的构效关系及制定精准营养策略提供依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biological Macromolecules
International Journal of Biological Macromolecules 生物-生化与分子生物学
CiteScore
13.70
自引率
9.80%
发文量
2728
审稿时长
64 days
期刊介绍: The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.
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