A binuclear zinc complex (Zn2L) as ratiometric probe for the pyrophosphate (PPi) sensing†

IF 2.7 3区化学 Q2 CHEMISTRY, ANALYTICAL

Analytical Methods Pub Date : 2025-03-24 DOI:10.1039/D5AY00028A

Mengxia Liu, Wenwen Sun, Le Wang, Zhiqiang Niu, Xiao Zhang, Dingjia Xiao, Tianjia Xiao and Junfeng Wang

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Abstract

Phosphate anion (PPi) as hydrolysis products of adenosine triphosphate (ATP), is related to various biochemical reactions, particularly energy storage and transfer, nucleotide metabolism, and cellular signaling. In this paper, a binuclear zinc complex (Zn₂L) was prepared for the PPi sensing in Tris–HCl solution. The results showed a clear fluorescence change from blue to green (425 nm → 500 nm) with the addition of PPi to the probe. The probe Zn₂L demonstrated a remarkable selectivity for PPi in comparison to common anions, with an estimated limit of detection (LOD) of 0.29 μM within a working range of 0–9 μM. UV titration experiments showed two binding steps from 1 : 1 to 1 : 2 complex (Zn₂L–PPi). Density-functional theory (DFT) studies were further conducted to find an energy gap that decreases with complex (probe + 2PPi) formation, resulting in a redshift of the absorption spectrum (360 nm → 430 nm). In addition, the Zn₂L probe was also used for PPi sensing in a urine sample, in which relative standard deviation values (1.19–2.17%) and recoveries (95.00–103.00%) were obtained.

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双核锌配合物（Zn2L）作为焦磷酸盐（PPi）传感的比例探针。

磷酸阴离子（PPi）作为三磷酸腺苷（ATP）的水解产物，与多种生化反应有关，尤其是能量的储存和传递、核苷酸代谢和细胞信号传导等。本文制备了一种双核锌配合物（Zn2L），用于在Tris-HCl溶液中检测PPi。结果表明，探针中加入PPi后，荧光从425 nm→500 nm由蓝色变为绿色。与普通阴离子相比，探针Zn2L对PPi具有显著的选择性，在0 ~ 9 μM的工作范围内，估计检出限（LOD）为0.29 μM。紫外滴定实验显示，配合物（Zn2L-PPi）从1:1到1:1有两个结合步骤。进一步进行密度泛函理论（DFT）研究发现，随着配合物（探针+ 2PPi）的形成，能隙减小，导致吸收光谱红移（360 nm→430 nm）。此外，还将Zn2L探针用于尿液样品的PPi检测，获得了相对标准偏差值（1.19 ~ 2.17%）和回收率（95.00 ~ 103.00%）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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