Single-cell RNA sequencing reveals a new mechanism of endothelial cell heterogeneity and healing in diabetic foot ulcers.

IF 5.7 2区 生物学 Q1 BIOLOGY
Songyun Zhao, Hua Yu, Zihao Li, Wanying Chen, Kaibo Liu, Hao Dai, Gaoyi Wang, Zibing Zhang, Jiaheng Xie, Yucang He, Liqun Li
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Abstract

Diabetic foot ulcers (DFU) are a common and severe complication among diabetic patients, posing a significant burden on patients' quality of life and healthcare systems due to their high incidence, amputation rates, and mortality. This study utilized single-cell RNA sequencing technology to deeply analyze the cellular heterogeneity of the skin on the feet ofDFU patients and the transcriptomic characteristics of endothelial cells, aiming to identify key cell populations and genes associated with the healing and progression of DFU. The study found that endothelial cells from DFU patients exhibited significant transcriptomic differences under various conditions, particularly in signaling pathways related to inflammatory responses and angiogenesis. Through trajectory analysis and cell communication research, we revealed the key role of endothelial cell subsets in the development of DFU and identified multiple important gene modules associated with the progression of DFU. Notably, the promoting effect of the SH3BGRL3 gene on endothelial cell proliferation, migration, and angiogenic capabilities under high glucose conditions was experimentally verified, providing a new potential target and theoretical basis for the treatment of DFU. This study not only enhances the understanding of the pathogenesis ofDFU but also provides a scientific basis for the development ofnew therapeutic strategies.

单细胞RNA测序揭示了糖尿病足溃疡内皮细胞异质性和愈合的新机制。
糖尿病足溃疡(DFU)是糖尿病患者中一种常见且严重的并发症,由于其高发病率、截肢率和死亡率,对患者的生活质量和医疗保健系统造成了重大负担。本研究利用单细胞RNA测序技术,深入分析DFU患者足部皮肤的细胞异质性和内皮细胞的转录组学特征,旨在鉴定与DFU愈合进展相关的关键细胞群和基因。研究发现,DFU患者的内皮细胞在各种情况下都表现出显著的转录组差异,特别是在炎症反应和血管生成相关的信号通路上。通过轨迹分析和细胞通讯研究,我们揭示了内皮细胞亚群在DFU发展中的关键作用,并确定了多个与DFU发展相关的重要基因模块。值得注意的是,实验证实了SH3BGRL3基因对高糖条件下内皮细胞增殖、迁移和血管生成能力的促进作用,为DFU的治疗提供了新的潜在靶点和理论基础。本研究不仅提高了对dfu发病机制的认识,而且为开发新的治疗策略提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology Direct
Biology Direct 生物-生物学
CiteScore
6.40
自引率
10.90%
发文量
32
审稿时长
7 months
期刊介绍: Biology Direct serves the life science research community as an open access, peer-reviewed online journal, providing authors and readers with an alternative to the traditional model of peer review. Biology Direct considers original research articles, hypotheses, comments, discovery notes and reviews in subject areas currently identified as those most conducive to the open review approach, primarily those with a significant non-experimental component.
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