Deletion of GhSCY2D Causes Impaired Chloroplast Development and Temperature-Dependent Leaf Yellowing in Cotton (Gossypium hirsutum L.).

Abstract

Leaf colour mutants play an important role in understanding chlorophyll metabolism and photosynthesis. In this study, we characterised a temperature-sensitive yellow leaf cotton mutant. Genome re-sequencing and comparison identified a 10.327 Kb deletion on the D12 chromosome (D12:670726-681053) in the mutant. The deletion region contains two annotated genes, GH_D12G0047 and GH_D12G0048. Investigations integrating gene mapping, comparative transcriptome analysis, gene annotation, virus-induced gene silencing and gene complementation, found deletion of GH_D12G0047 or GhSCY2D, a crucial constituent of the Sec2 complex essential for the function of chloroplasts, being responsible for the yellow leaf phenotype. The yellow leaf mutant had disrupted chloroplast structure and hindered chlorophyll synthesis when temperature was below 28°C but regained normal green leaf colour at 32°C. By analysing the transcriptome data and hormonal level changes of the mutant under conditions of 25°C and 32°C, it was found that the jasmonic acid signalling pathway and GhSCY2 work in concert to maintain the structural integrity of chloroplasts. The outcomes of the study reveal the indispensable role of GhSCY2 and jasmonic acid in sustaining chloroplast homoeostasis, providing new insights into the regulation of cotton leaf colour and paving the way for advancement in high photosynthetic efficiency breeding strategies.