Mitigating Bisphenol-Induced Neurotoxicity: Exploring the Therapeutic Potential of Diosmin in Zebrafish Larvae

IF 3.9 3区医学 Q2 FOOD SCIENCE & TECHNOLOGY

Food and Chemical Toxicology Pub Date : 2025-03-21 DOI:10.1016/j.fct.2025.115402

Deenathayalan Uvarajan , Manish Ravikumar , Brindha Durairaj

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Abstract

Neurological disorders are commonly accompanied by inflammation of the brain, which can be triggered by oxidative stress and cell damage caused by hazardous environmental substances. The ubiquitous harmful chemical bisphenol A (BPA) has been linked to several neuropsychiatric disorders and is thought to contribute to oxidative damage. This study explored the mechanisms underlying the effects of BPA on neurological health. Diosmin (DM) is a natural flavonoid (C₂₈H₃₂O₁₅) found in various plants, including citrus fruits and it possess various pharmacological activities. This study investigated the neuroprotective effects of DM on BPA-induced neuroinflammation in zebrafish larvae, suggesting its potential therapeutic uses. Developmental toxicity, including mortality, hatching rate, and heart rate, was evaluated to determine DM toxicity. Oxidative stress biomarkers such as reactive oxygen species (ROS), superoxide anions (O⁻²), lipid peroxidation (LPO), and nitric oxide (NO) were quantified using colorimetric assays in the head region of the larvae. Antioxidant enzyme activities were measured to assess the impact of DM on antioxidant defences. Neuroinflammation was evaluated by analysing pro-inflammatory markers using RT-qPCR, and motor neuron function was assessed using acetylcholinesterase (AChE) activity and behavioural assays. The findings indicate that exposure to DM prevents neurotoxicity induced by BPA by increasing antioxidant defence enzymes and reducing the levels of ROS, O²⁻, LPO, and NO in the head region of zebrafish larvae. Furthermore, DM enhanced motor neuron function by increasing AChE activity and decreasing neuroinflammation by reducing the levels of pro-inflammatory markers influenced by BPA. This study suggests that DM offers neuroprotection against BPA-induced oxidative damage and neuroinflammation, thereby paving the way for the development of new treatment options for neurological disorders.

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减轻双酚诱导的神经毒性：探索地奥米明对斑马鱼幼虫的治疗潜力。

神经系统疾病通常伴有大脑炎症，这可能由氧化应激和有害环境物质引起的细胞损伤引发。普遍存在的有害化学物质双酚A （BPA）与几种神经精神疾病有关，并被认为会导致氧化损伤。本研究探讨了BPA对神经系统健康影响的潜在机制。薯蓣皂苷（dismin， DM）是一种天然类黄酮（C28H32O15），存在于包括柑橘类水果在内的多种植物中，具有多种药理活性。本研究探讨了DM对bpa诱导的斑马鱼幼鱼神经炎症的神经保护作用，提示其潜在的治疗用途。评估发育毒性，包括死亡率、孵化率和心率，以确定DM毒性。用比色法测定了氧化应激生物标志物，如活性氧（ROS）、超氧阴离子（O-2）、脂质过氧化（LPO）和一氧化氮（NO）。通过测定抗氧化酶活性来评估DM对抗氧化防御的影响。通过RT-qPCR分析促炎标志物来评估神经炎症，通过乙酰胆碱酯酶（AChE）活性和行为分析来评估运动神经元功能。研究结果表明，暴露于DM可通过增加抗氧化防御酶和降低斑马鱼头部区域的ROS、O2-、LPO和NO水平来预防BPA诱导的神经毒性。此外，DM通过增加AChE活性增强运动神经元功能，并通过降低BPA影响的促炎标志物水平来减少神经炎症。这项研究表明，DM对bpa诱导的氧化损伤和神经炎症具有神经保护作用，从而为开发新的神经疾病治疗方案铺平了道路。

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来源期刊

Food and Chemical Toxicology 工程技术-毒理学

CiteScore

10.90

自引率

4.70%

发文量

651

审稿时长

31 days

期刊介绍： Food and Chemical Toxicology (FCT), an internationally renowned journal, that publishes original research articles and reviews on toxic effects, in animals and humans, of natural or synthetic chemicals occurring in the human environment with particular emphasis on food, drugs, and chemicals, including agricultural and industrial safety, and consumer product safety. Areas such as safety evaluation of novel foods and ingredients, biotechnologically-derived products, and nanomaterials are included in the scope of the journal. FCT also encourages submission of papers on inter-relationships between nutrition and toxicology and on in vitro techniques, particularly those fostering the 3 Rs. The principal aim of the journal is to publish high impact, scholarly work and to serve as a multidisciplinary forum for research in toxicology. Papers submitted will be judged on the basis of scientific originality and contribution to the field, quality and subject matter. Studies should address at least one of the following: -Adverse physiological/biochemical, or pathological changes induced by specific defined substances -New techniques for assessing potential toxicity, including molecular biology -Mechanisms underlying toxic phenomena -Toxicological examinations of specific chemicals or consumer products, both those showing adverse effects and those demonstrating safety, that meet current standards of scientific acceptability. Authors must clearly and briefly identify what novel toxic effect (s) or toxic mechanism (s) of the chemical are being reported and what their significance is in the abstract. Furthermore, sufficient doses should be included in order to provide information on NOAEL/LOAEL values.