A Rapid Heat-Enhanced Golgi-Cox Staining Method for Detailed Neuroanatomical Analysis Coupled With Immunostaining

IF 2.3 4区医学 Q3 NEUROSCIENCES

Journal of Comparative Neurology Pub Date : 2025-03-24 DOI:10.1002/cne.70042

Da-Jiang Hui, Mei-Xue Yuan, Xin-Ya Qin, An-Qi Zhang, Chen-Wei Wang, Yu Wang, Jiang-Ning Zhou, Peng Chen, Qing-Hong Shan

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Abstract

The Golgi-Cox staining technique is renowned for its ability to delineate neuronal architecture with remarkable precision. However, the traditional protocol's lengthy processing timeline and limited compatibility with immunostaining and transgenic labeling have hindered its widespread adoption in modern neuroscience research.

In the current study, we found that adjusting the incubation temperature to 55°C significantly reduced the staining duration to a mere 24 h for 100 µm-thick sections of mouse brain tissue. Importantly, our optimized protocol is compatible with immunostaining techniques and transgenic mouse models. In addition, using a lipopolysaccharides-induced mouse model of depression, we found a reduction in dendritic spines labeled by Golgi-Cox staining and an increase in the number of microglial cells labeled by immunofluorescence in the same samples, in addition, cross-talk between Golgi-Cox-stained neurons and microglial fibers were observed.

In conclusion, the modified Golgi-Cox staining technique allows for the acquisition of a more comprehensive set of data from the same biological tissue with increased efficiency. This advancement promises to improve methodologies in histopathology and neurobiology, making advanced applications of Golgi-Cox staining more accessible in contemporary neuroscience research.

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来源期刊

Journal of Comparative Neurology 医学-动物学

CiteScore

5.80

自引率

8.00%

发文量

158

审稿时长

3-6 weeks

期刊介绍： Established in 1891, JCN is the oldest continually published basic neuroscience journal. Historically, as the name suggests, the journal focused on a comparison among species to uncover the intricacies of how the brain functions. In modern times, this research is called systems neuroscience where animal models are used to mimic core cognitive processes with the ultimate goal of understanding neural circuits and connections that give rise to behavioral patterns and different neural states. Research published in JCN covers all species from invertebrates to humans, and the reports inform the readers about the function and organization of nervous systems in species with an emphasis on the way that species adaptations inform about the function or organization of the nervous systems, rather than on their evolution per se. JCN publishes primary research articles and critical commentaries and review-type articles offering expert insight in to cutting edge research in the field of systems neuroscience; a complete list of contribution types is given in the Author Guidelines. For primary research contributions, only full-length investigative reports are desired; the journal does not accept short communications.