Exploring the Mechanism of Action of Honeybran-Fried Cimicifuga Rhizoma in the Treatment of IBS-D Based on Metabolomics and Network Pharmacology

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-03-24 DOI:10.1002/bmc.70026

Jing Zhu, Yuxuan Zou, Yigeng Wu, Xiaoxia Deng, Yiming Huang, En Yuan, Qi Chen

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引用次数: 0

Abstract

Honeybran-fried Cimicifuga Rhizoma (HBCR) is often used to treat prolonged diarrhea and prolapse of the anus, uterine prolapse, and gastric ptosis caused by spleen qi deficiency and the inability to elevate qi, and thus the lowering of middle qi. Rats were divided randomly into four groups. Fecal samples of rats in each group were subjected to metabolomics analysis. We identified the chemical components of HBCR using liquid chromatography–tandem mass spectrometry. We predicted the potential active components and key targets of HBCR using network pharmacology to construct a “drug–potential active ingredient–target–disease” network. The key targets screened by network pharmacology and differential metabolites screened by metabolomics analysis were subjected to combined pathway analysis. Pharmacodynamic indices showed that HBCR had a good therapeutic effect upon IBS-D. Metabolomics analysis revealed 26 differential metabolites in the treatment of IBS-D by HBCR. A total of 69 chemical components were identified, and 32 potential active components and 296 key targets were screened. Combination of metabolomics analysis and network pharmacology for joint pathway analysis revealed that the therapeutic effect of HBCR may be affected by the metabolism of linoleic acid, retinol, arachidonic acid, and tryptophan. HBCR had significant therapeutic effects in rats with IBS-D.

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基于代谢组学和网络药理学探讨蜂蜜炒慈母治疗IBS-D的作用机制

银杏炒慈母常用于治疗因脾虚不能升气而引起的长时间腹泻、肛门脱垂、子宫脱垂、胃下垂等中气降症。将大鼠随机分为四组。对各组大鼠粪便进行代谢组学分析。我们用液相色谱-串联质谱法鉴定了HBCR的化学成分。利用网络药理学对HBCR的潜在有效成分和关键靶点进行预测，构建“药物潜在有效成分-靶点-疾病”网络。对网络药理学筛选的关键靶点和代谢组学分析筛选的差异代谢物进行联合通路分析。药效学指标显示HBCR对IBS-D有良好的治疗效果。代谢组学分析显示，HBCR治疗IBS-D有26种差异代谢物。共鉴定出69个化学成分，筛选出32个潜在有效成分和296个关键靶点。结合代谢组学分析和网络药理学联合通路分析发现，HBCR的治疗效果可能受亚油酸、视黄醇、花生四烯酸和色氨酸代谢的影响。HBCR对IBS-D大鼠有显著的治疗作用。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.