Islam M. Mostafa, Mahmoud A. Omar, Mohamed Ahmed Elsayed, Abobakr A. Mohamed
{"title":"Hantzsch Reaction–Based Fluorimetric Approach for Sensitive Determination of Besifloxacin in Pharmaceutical Eye Drops and Aqueous Humor","authors":"Islam M. Mostafa, Mahmoud A. Omar, Mohamed Ahmed Elsayed, Abobakr A. Mohamed","doi":"10.1002/bio.70154","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>An innovative and sensitive analytical tool (fluorimetric spectroscopy) for quantifying besifloxacin (BFC) in eye drops and aqueous humor is introduced. The method presented here utilizes a well-known derivatization reaction called Hantzsch reaction to activate BFC's fluorescence. All the factors affecting the sensitivity of the developed approach were optimized to boost the method's sensitivity. After optimizations, this reaction enables the fluorimetric determination (at 485 nm) of BFC in eye drops and spiked aqueous humor without any obstructions from the naturally present biointerferents. The dynamic range for BFC determination in the pure state is 0.15–1.0 (μg/mL), with detection and quantification limits of 0.02 and 0.05 (μg/mL), respectively. The approach exhibits favorable recovery rates in spiked aqueous humor (95.5% ± 1.15%–100.3% ± 0.50%).</p>\n </div>","PeriodicalId":49902,"journal":{"name":"Luminescence","volume":"40 3","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Luminescence","FirstCategoryId":"92","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bio.70154","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
An innovative and sensitive analytical tool (fluorimetric spectroscopy) for quantifying besifloxacin (BFC) in eye drops and aqueous humor is introduced. The method presented here utilizes a well-known derivatization reaction called Hantzsch reaction to activate BFC's fluorescence. All the factors affecting the sensitivity of the developed approach were optimized to boost the method's sensitivity. After optimizations, this reaction enables the fluorimetric determination (at 485 nm) of BFC in eye drops and spiked aqueous humor without any obstructions from the naturally present biointerferents. The dynamic range for BFC determination in the pure state is 0.15–1.0 (μg/mL), with detection and quantification limits of 0.02 and 0.05 (μg/mL), respectively. The approach exhibits favorable recovery rates in spiked aqueous humor (95.5% ± 1.15%–100.3% ± 0.50%).
期刊介绍:
Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry.
Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.