Hantzsch Reaction–Based Fluorimetric Approach for Sensitive Determination of Besifloxacin in Pharmaceutical Eye Drops and Aqueous Humor

IF 3.2 4区 化学 Q2 CHEMISTRY, ANALYTICAL
Luminescence Pub Date : 2025-03-24 DOI:10.1002/bio.70154
Islam M. Mostafa, Mahmoud A. Omar, Mohamed Ahmed Elsayed, Abobakr A. Mohamed
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引用次数: 0

Abstract

An innovative and sensitive analytical tool (fluorimetric spectroscopy) for quantifying besifloxacin (BFC) in eye drops and aqueous humor is introduced. The method presented here utilizes a well-known derivatization reaction called Hantzsch reaction to activate BFC's fluorescence. All the factors affecting the sensitivity of the developed approach were optimized to boost the method's sensitivity. After optimizations, this reaction enables the fluorimetric determination (at 485 nm) of BFC in eye drops and spiked aqueous humor without any obstructions from the naturally present biointerferents. The dynamic range for BFC determination in the pure state is 0.15–1.0 (μg/mL), with detection and quantification limits of 0.02 and 0.05 (μg/mL), respectively. The approach exhibits favorable recovery rates in spiked aqueous humor (95.5% ± 1.15%–100.3% ± 0.50%).

基于Hantzsch反应的荧光法灵敏测定药用滴眼液和体液中贝西沙星的含量
介绍了一种新颖、灵敏的分析工具(荧光光谱法),用于定量眼药水和房水中的贝西沙星(BFC)。本文提出的方法利用一种众所周知的衍生化反应,称为汉奇反应来激活BFC的荧光。对影响方法灵敏度的因素进行了优化,提高了方法的灵敏度。经过优化后,该反应能够在不受天然存在的生物干扰的情况下,用荧光法(在485 nm)测定滴眼液和加标体液中的BFC。BFC纯态测定的动态范围为0.15 ~ 1.0 (μg/mL),检测限为0.02,定量限为0.05 (μg/mL)。该方法在加标房水中具有良好的回收率(95.5%±1.15% ~ 100.3%±0.50%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Luminescence
Luminescence 生物-生化与分子生物学
CiteScore
5.10
自引率
13.80%
发文量
248
审稿时长
3.5 months
期刊介绍: Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.
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