Acute Stress Effects Over Time on the Stress Axis in the Carp (Cyprinus carpio) Brain

IF 1.9 4区 农林科学 Q2 FISHERIES
Constanze Pietsch, Paulina Pawlak, Jonathan Konrad
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Abstract

This study investigates gene expression profiles over time in the brain of juvenile carp subjected to several acute stressors, which have not yet been investigated in such detail in this fish species so far. A stress trial with carp was conducted aiming at identifying relevant differences in gene expressions 30, 60, and 90 min after treatment. Using quantitative real-time PCR, the expression of hypothalamic–pituitary–interrenal (HPI)-related axis genes and immediate early genes (IEGs) across four different brain parts (telencephalon, hypothalamus, optic tectum, rhombencephalon). Our findings show that acute stress resulted in pronounced effects on the gene expression patterns in all brain parts between control fish or fish sampled 30, 60, or 90 min after stress application. Furthermore, the gene regulation patterns in feed rewarded and feed control fish frequently show differences to the gene expression patterns of confined and air-exposed fish. These results provide new insights into the dynamics of stress-induced gene regulation in fish, emphasizing the complexity and specificity of the HPI axis response to different stressors over time.

Abstract Image

本研究调查了幼年鲤鱼大脑在遭受几种急性应激后随时间变化的基因表达谱。对鲤鱼进行了应激试验,旨在确定处理后 30、60 和 90 分钟内基因表达的相关差异。利用定量实时 PCR 技术,研究了下丘脑-垂体-肾上腺(HPI)相关轴基因和即时早期基因(IEGs)在四个不同脑部(端脑、下丘脑、视神经构造、菱形脑)的表达情况。我们的研究结果表明,急性应激对对照组鱼类或应激后30、60或90分钟采样的鱼类大脑各部位的基因表达模式都有明显影响。此外,饲料奖励鱼和饲料控制鱼的基因调控模式经常与密闭鱼和空气暴露鱼的基因表达模式不同。这些结果为鱼类应激诱导基因调控的动态变化提供了新的见解,强调了HPI轴对不同应激源的反应随时间变化的复杂性和特异性。
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来源期刊
Aquaculture Research
Aquaculture Research 农林科学-渔业
CiteScore
4.60
自引率
5.00%
发文量
464
审稿时长
5.3 months
期刊介绍: International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.
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