Integrating Metabolic RNA Labeling-Based Time-Resolved Single-Cell RNA Sequencing with Spatial Transcriptomics for Spatiotemporal Transcriptomic Analysis (Small Methods 3/2025)

IF 10.7 2区材料科学 Q1 CHEMISTRY, PHYSICAL

Small Methods Pub Date : 2025-03-20 DOI:10.1002/smtd.202570019

Xiaoyong Chen, Shichao Lin, Honghai You, Jinyuan Chen, Qiaoyi Wu, Kun Yin, Fanghe Lin, Yingkun Zhang, Jia Song, Chenyu Ding, Dezhi Kang, Chaoyong Yang

引用次数: 0

Abstract

Inside Back Cover

In article number 2401297, Ding, Kang, Yang, and co-workers integrate metabolic RNA labeling-based time-resolved scRNA-seq with spatial transcriptomics (10x Visium and in situ sequencing) to profile the transcriptional dynamics of glioblastoma cells. The spatiotemporal analysis elucidates the complex gene regulatory mechanism of EZH2-mediated mesenchymal transition in the tumor microenvironment.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Small Methods Materials Science-General Materials Science

CiteScore

17.40

自引率

1.60%

发文量

347

期刊介绍： Small Methods is a multidisciplinary journal that publishes groundbreaking research on methods relevant to nano- and microscale research. It welcomes contributions from the fields of materials science, biomedical science, chemistry, and physics, showcasing the latest advancements in experimental techniques. With a notable 2022 Impact Factor of 12.4 (Journal Citation Reports, Clarivate Analytics, 2023), Small Methods is recognized for its significant impact on the scientific community. The online ISSN for Small Methods is 2366-9608.