Increase in the Intensity of Free Radical Processes of Tumor Cells by Silver Bisilicate Nanoparticles

Abstract

The chemiluminescence intensity (CI) when normal and tumor cells of humans are exposed to nanostructured silver bisilicate Ag₆Si₂O₇ (silver atomic clusters, SACs) are measured. The following cell types are used: skin fibroblasts F022, mesenchymal stromal cells (MSCs) M032, multiple myeloma cells RPMI8226, and acute myelogenous leukemia cells KG1. The chemiluminescence parameters (total light sum (S) and the maximal light intensity (I_max)) of normal cells increase by 1.4–1.5 times after adding SACs compared to the reference values regardless of the SAC concentration. The S and I_max values for tumor cells are between 2.1 and 2.8 times higher than the reference values depending on the SACs dose. In the reference data the tumor cells show a higher slope ratio of the trailing edge of radiation pulse of the CI decrease curve (1.02–1.10) than the normal cells (0.67–0.72). SACs with a concentration of 2.0–3.0 µg/mL significantly decrease tumor cell viability and do not affect normal cells. A higher sensitivity to SACs corresponds to a higher CI level in the cells.