Effect of a Bacillus-based probiotic on fecal shedding and cecal colonization of Salmonella Enteritidis in laying hens

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
I. Poudel , A. Calvert , A.S. Kiess , L. Zhang , P.A. Adhikari
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引用次数: 0

Abstract

This study was conducted to evaluate a Bacillus-based probiotic to reduce Salmonella Enteritidis (SE) infection in Hy-Line W-36 hens at 24 weeks of age. The hens were assigned to four treatment groups: non challenged with basal diet (NC); challenged with basal diet (PC), PC + probiotic at 1 × 106 CFU/kg (PRO1), and PC + probiotic at 2 × 106 CFU /kg (PRO2). The probiotic used was a combination of five strains of Bacillus spp. Birds were adapted to the experimental diets for one week after which challenged birds were orally gavaged for two consecutive days with approximately 1 × 109 colony-forming units (CFU) of nalidixic acid-resistant SE. Feed and water were provided ad libitum throughout the experiment. Fecal samples were collected on 3-, 7- and 14-days post inoculation (dpi). Cecal contents were collected aseptically on 7 and 14 dpi. Both fecal and cecal samples were plated on xylose lysine tergitol 4 (XLT4) plates with 200 ppm nalidixic acid using the direct plate method. Fecal samples were further enriched as per the National Poultry Improvement Plan procedures on tetrathionate (TT) and Rappaport Vassiliadis (RV) broth. Data were log-transformed and analyzed using the PROC GLIMMIX procedure of SAS 9.4. In the Ceca, PRO1 reduced the concentration of SE by 0.90 logs at 7 dpi (P=0.046). Although at 14 dpi both PRO1 and PRO2 decreased SE by 0.40 and 0.60 logs in the ceca (P=0.08). Similarly at 14 dpi, PRO1 and PRO2 reduced SE concentration by 0.80 and 0.75 logs in fecal samples using direct plating (P=0.032). Similarly, we observed that RV and TT enrichment increased SE counts by 1.5 and 2.6 logs, respectively at 14 dpi (P<0.001). This indicates that the enrichment method increased the SE enumeration as compared to direct plating method. There were no differences in SE fecal shedding at 0, 3, and 7 dpi by direct platting or enrichment methods. In conclusion, Bacillus-based probiotics have the potential to reduce SE in both ceca and fecal samples from laying hens.
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来源期刊
Journal of Applied Poultry Research
Journal of Applied Poultry Research 农林科学-奶制品与动物科学
CiteScore
4.10
自引率
10.50%
发文量
80
审稿时长
104 days
期刊介绍: The Journal of Applied Poultry Research (JAPR) publishes original research reports, field reports, and reviews on breeding, hatching, health and disease, layer management, meat bird processing and products, meat bird management, microbiology, food safety, nutrition, environment, sanitation, welfare, and economics. As of January 2020, JAPR will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. The readers of JAPR are in education, extension, industry, and government, including research, teaching, administration, veterinary medicine, management, production, quality assurance, product development, and technical services. Nutritionists, breeder flock supervisors, production managers, microbiologists, laboratory personnel, food safety and sanitation managers, poultry processing managers, feed manufacturers, and egg producers use JAPR to keep up with current applied poultry research.
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