The role of galactose and chitosan in novel targeted nanoemulsion delivery carriers: Synthesis, in vitro stability, and anti-Hepa 1–6 cell activity

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2025-03-18 DOI:10.1016/j.carbpol.2025.123515

Yaqing Fan , Huasong Bai , Tong Liu , Ruixue Wang , Zhanzhong Wang

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引用次数: 0

Abstract

This study developed water-in-oil-in-water (W/O/W) nanoemulsions (NEs) modified with galactose (GAL) and chitosan (CTS) to encapsulate α-linolenic acid (ALA) for targeted delivery. The evaluation of physicochemical properties, stability, release characteristics, and in vitro targeting explored the effect of adding GAL to the external aqueous phase, as well as the interaction between GAL and CTS, on the targeted delivery performance of the NEs. The CTS and GAL maintained NE structural stability and thermal resistance through electrostatic interactions, preserving a stable encapsulation rate. The CTS in the external aqueous phase facilitated a three-dimensional network, maintaining flow stability. The in vitro digestion showed <22 % ALA release in gastric fluid and <45 % in intestinal fluid within 2 h. Additionally, in vitro cell experiments showed that NEs increased lipid oxidation, decreased superoxide dismutase activity, and increased lactate dehydrogenase release. The NEs with GAL added to the external aqueous phase demonstrated high uptake in Hepa 1–6 cells, indicating effective liver targeting. Thus, NEs with GAL and CTS could serve as carriers for active compounds, offering a novel strategy for targeted nutrient delivery in the food and pharmaceutical industries.

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本研究开发了用半乳糖（GAL）和壳聚糖（CTS）改性的水包油型（W/O/W）纳米乳液（NEs），用于包裹α-亚麻酸（ALA）进行靶向递送。通过对理化性质、稳定性、释放特性和体外靶向性的评估，探讨了在外部水相中添加 GAL 以及 GAL 与 CTS 之间的相互作用对 NEs 靶向递送性能的影响。CTS 和 GAL 通过静电作用保持了 NE 的结构稳定性和耐热性，从而保持了稳定的包封率。外部水相中的 CTS 有助于形成三维网络，保持流动稳定性。此外，体外细胞实验表明，NEs 增加了脂质氧化，降低了超氧化物歧化酶的活性，增加了乳酸脱氢酶的释放。在外部水相中加入 GAL 的 NEs 在 Hepa 1-6 细胞中的吸收率很高，这表明 NEs 能有效地靶向肝脏。因此，含有 GAL 和 CTS 的 NEs 可作为活性化合物的载体，为食品和制药行业的定向营养输送提供了一种新策略。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.