Diagnosis of Cladophialophora bantiana cerebral infection by clinical metagenomics

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2025-03-20 DOI:10.1016/j.diagmicrobio.2025.116822

Wanessa Rolando Roselli , Fernando Franco de Oliveira Miraglia , Fernanda de Mello Malta , Gustavo Bruniera Peres Fernandes , Guilherme Carvalhal Ribas , Clécio de Oliveira Godeiro Júnior , Deyvid Emanuel Amgarten , Fabiane Camargo G Nunes , Mario Trindade , João Nóbrega de Almeida Junior , Marines Dalla Valle Martino , Rubia Anita Ferraz Santana , João Renato Rebello Pinho , André Mario Doi

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引用次数: 0

Abstract

Purpose

Cladophialophora bantiana is a rare cause of severe central nervous system phaeohyphomycosis.

Results

A 44-year-old woman from Northeastern Brazil with a history of scotomas, mental confusion, and impaired coordination presented Magnetic Resonance Imaging with vasogenic edema and nodular enhancement in the left occipital lobe. Brain biopsy showed neutrophilic exudate with eosinophils, macrophages, giant multinucleated cells, and septate hyphae with a brown-colored birefringent wall and acute angle branching. Metagenomic analysis of the biopsy's total RNA revealed the presence of RNA sequences highly similari to C. bantiana. Culture confirmed the presence of olivaceous-gray suede-like to floccose colonies, with septate dematiaceous hyphae and long conidia chains from undifferentiated conidiophores, consistent with C. bantiana.

Conclusions

This case highlights the potential of metagenomic testing as a tool for early diagnosis of infections caused by uncommon fungal pathogens. To our knowledge, this is the first report of C. bantiana central nervous system infection in Brazil.

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.