Preparation of TPM–NCs–gel and its effect on subcutaneous abscess caused by Staphylococcus aureus

Quanwei Xie , Feirong Zhou , Runan He , Lianbao Ye , Zonghao Lin , Xiangyu Nie , Yuanzheng Wei , Chuqin Yu
{"title":"Preparation of TPM–NCs–gel and its effect on subcutaneous abscess caused by Staphylococcus aureus","authors":"Quanwei Xie ,&nbsp;Feirong Zhou ,&nbsp;Runan He ,&nbsp;Lianbao Ye ,&nbsp;Zonghao Lin ,&nbsp;Xiangyu Nie ,&nbsp;Yuanzheng Wei ,&nbsp;Chuqin Yu","doi":"10.1016/j.jhip.2025.02.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><div>The insoluble compound 1,1′-(2,4,6-trihydroxy-1,3-phenylene)bis(3-methylbutan-1-one) (TPM) is used in preparing a TPM nanocrystals gel (TPM–NCs–gel), and its <em>in vitro</em> antibacterial activity and therapeutic effect on subcutaneous abscesses caused by <em>Staphylococcus aureus</em> were evaluated.</div></div><div><h3>Methods</h3><div>The effect of a prescription technology on the particle size of a TPM–NCs suspension was investigated using a single factor, and the TPM–NCs prescription was optimized using a Box–Behnken design. A TPM–NCs–gel was prepared using hydroxyethyl cellulose–HHX (HEC–HHX) as a gel matrix and characterized. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of TPM–NCs–gel were determined with the microdilution method. The susceptibility of <em>Staphylococcus aureus</em> to mupirocin ointment, TPM–NCs–gel, and TPM–gel was evaluated by the disk diffusion method. The efficacy of the TPM–NCs–gel for subcutaneous abscess caused by <em>Staphylococcus aureus</em> was evaluated using a mouse model.</div></div><div><h3>Results</h3><div>The optimized TPM–NCs prescription consisted of Tween 80 and TPGS (2.47%), TPM (1.24%) and mannitol (2.32%). The size of the TPM–NCs was 98.2 ​± ​3.9 ​nm, and the polydispersion coefficient (PDI) was 0.235 ​± ​0.023. The particle size and PDI of the TPM–NCs–gel were 112.4 ​± ​7.3 ​nm and 0.148 ​± ​0.068, respectively. The MIC and MBC were both 2.98 ​μg/mL. After 12 days of administration, the bacteria in the abscess site of 2% TPM–NCs–gel experimental group were cleared, the inflammatory cells were reduced, and the skin structure was remodeled.</div></div><div><h3>Conclusion</h3><div>After TPM was prepared into TPM–NCs–gel, the antibacterial activity was enhanced, <em>Staphylococcus aureus</em> at the site of abscess was effectively removed, and wound healing was promoted.</div></div>","PeriodicalId":100787,"journal":{"name":"Journal of Holistic Integrative Pharmacy","volume":"6 1","pages":"Pages 41-49"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Holistic Integrative Pharmacy","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2707368825000044","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Objective

The insoluble compound 1,1′-(2,4,6-trihydroxy-1,3-phenylene)bis(3-methylbutan-1-one) (TPM) is used in preparing a TPM nanocrystals gel (TPM–NCs–gel), and its in vitro antibacterial activity and therapeutic effect on subcutaneous abscesses caused by Staphylococcus aureus were evaluated.

Methods

The effect of a prescription technology on the particle size of a TPM–NCs suspension was investigated using a single factor, and the TPM–NCs prescription was optimized using a Box–Behnken design. A TPM–NCs–gel was prepared using hydroxyethyl cellulose–HHX (HEC–HHX) as a gel matrix and characterized. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of TPM–NCs–gel were determined with the microdilution method. The susceptibility of Staphylococcus aureus to mupirocin ointment, TPM–NCs–gel, and TPM–gel was evaluated by the disk diffusion method. The efficacy of the TPM–NCs–gel for subcutaneous abscess caused by Staphylococcus aureus was evaluated using a mouse model.

Results

The optimized TPM–NCs prescription consisted of Tween 80 and TPGS (2.47%), TPM (1.24%) and mannitol (2.32%). The size of the TPM–NCs was 98.2 ​± ​3.9 ​nm, and the polydispersion coefficient (PDI) was 0.235 ​± ​0.023. The particle size and PDI of the TPM–NCs–gel were 112.4 ​± ​7.3 ​nm and 0.148 ​± ​0.068, respectively. The MIC and MBC were both 2.98 ​μg/mL. After 12 days of administration, the bacteria in the abscess site of 2% TPM–NCs–gel experimental group were cleared, the inflammatory cells were reduced, and the skin structure was remodeled.

Conclusion

After TPM was prepared into TPM–NCs–gel, the antibacterial activity was enhanced, Staphylococcus aureus at the site of abscess was effectively removed, and wound healing was promoted.
tpm - ncs凝胶的制备及其对金黄色葡萄球菌所致皮下脓肿的治疗作用
目的用不溶性化合物1,1′-(2,4,6-三羟基-1,3-苯基)双(3-甲基丁烷-1- 1)(TPM)制备TPM纳米晶凝胶(TPM - ncs -凝胶),并评价其体外抗菌活性和对金黄色葡萄球菌皮下脓肿的治疗效果。方法采用单因素法考察处方工艺对TPM-NCs混悬液粒径的影响,采用Box-Behnken设计优化TPM-NCs的处方。以羟乙基纤维素- hhx (HEC-HHX)为凝胶基质制备了tpm - ncs -凝胶,并对其进行了表征。采用微量稀释法测定了tpm - ncs -凝胶的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。采用纸片扩散法评价金黄色葡萄球菌对莫匹罗星软膏、tpm - ncs -凝胶和tpm -凝胶的敏感性。采用小鼠模型评价tpm - ncs -凝胶治疗金黄色葡萄球菌皮下脓肿的疗效。结果优选的Tween 80 + TPGS(2.47%)、TPM(1.24%) +甘露醇(2.32%)。tpm - nc的尺寸为98.2±3.9 nm, PDI为0.235±0.023。tpm - ncs -凝胶的粒径为112.4±7.3 nm, PDI为0.148±0.068。MIC和MBC均为2.98 μg/mL。给药12 d后,2% tpm - ncs凝胶试验组脓肿部位细菌清除,炎症细胞减少,皮肤结构重塑。结论将TPM制备成TPM - ncs -凝胶后,抗菌活性增强,脓肿部位金黄色葡萄球菌被有效清除,促进伤口愈合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信