Chemical characterization, anti-cholinesterase and insecticidal activities of Moroccan Artemisia absinthium L. leaf extracts against Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae)
Imane Naimi , Maroua Ait Tastift , Manal Zefzoufi , Chemseddoha Gadhi , Touria Ba M'hamed , Hafida Bouamama
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引用次数: 0
Abstract
The present investigation assessed the effect of solvent extraction (ethanol, ethyl acetate, and acetone) of Artemisia absinthium L. leaves on the chemical profile, total phenolic and flavonoid contents, anti-cholinesterase activity, and contact toxicity against T. castaneum adults. The extraction yield showed that ethanol extract was the most effective solvent for extraction, resulting in the highest extraction yield (5,74 %). The HPLC analysis results showed that the main components identified were 5-desmethylsinensetin (34,7 %), quercetin-dimethyl ester-O-hexoside (15,95 %), quercetin-tetramethyl ester (7,32 %), and apigenin 6-C-glucoside 8-C-arabinoside isomer 2 (6,52 %). This extract recorded the highest total phenolic and flavonoid content, with a value of 2,91 ± 0.08 mg GAE/g extract and 1,13 ± 0,02 mg QE/g extract, respectively. The ethanol extract caused high mortality rates of 93,3 % of T. castaneum adults at the concentration of 95 mg/mL after 72 h of exposure due to its high acetylcholinesterase enzyme inhibitory potential (19,98 ± 0,10 mg/ml). A. absinthium L. extract can be an alternative to chemical insecticides to control T. castaneum.
研究了不同溶剂(乙醇、乙酸乙酯和丙酮)对苦艾叶化学成分、总酚和类黄酮含量、抗胆碱酯酶活性以及对成虫接触毒性的影响。结果表明,乙醇提取物是最有效的提取溶剂,提取率最高(5.74%)。HPLC分析结果显示,其主要成分为5-去甲基青木苷(34,7%)、槲皮素-二甲酯- o -己糖苷(15,95%)、槲皮素-四甲基酯(7,32%)和芹菜素6- c -葡萄糖苷8- c -阿拉伯糖苷异构体2(6,52%)。总酚和总黄酮含量最高,分别为2.91±0.08 mg GAE/g和1.13±0.02 mg QE/g。乙醇提取物对乙酰胆碱酯酶抑制电位(19.98±0.10 mg/mL)高,在浓度为95 mg/mL时,暴露72 h后死亡率高达93.3%。苦艾草提取物可作为化学杀虫剂的替代品来防治栗霉病。
期刊介绍:
The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.