MnCoO nanosheets with enhanced oxidase mimetic activity: A novel platform for sensitive rutin detection

Abstract

This study introduces a new spectrophotometric approach for rutin quantitation using a smart platform based on the metal organic framework. Bimetallic porous Mn/Co oxide nanosheets (MnCoO-NSs) were synthesized through a simple, one-step hydrothermal process under mild conditions. A versatile “one-stone-two-birds” strategy was employed to create nanosheets rich in oxygen vacancies. These vacancies facilitate the activation of O2, generating the primary reactive intermediate, O2^•− radicals. This is followed by enhanced oxidase mimetic activity commencing 3,3′,5,5′-tetramethyl-benzidine (TMB) oxidation. Rutin could induce the reduction of oxTMB and result in a noticeable blue color fading. The prepared. NSs show high sensitivity towards rutin detection with a low detection limit of 0.25 µg mL⁻¹, along with satisfactory selectivity and reliability. The linearity range was 1.0–60.0 µg mL⁻¹. This research not only introduces an efficient, non-precious metal oxidase mimic but also develops a sensitive and environmentally friendly colorimetric approach for rutin determination. The green attributes of this method are substantiated through a comprehensive evaluation, highlighting its potential for sustainable analytical applications.