Super-resolution optical microscope based on a water-immersion metalens

IF 3.5 2区工程技术 Q2 OPTICS

Optics and Lasers in Engineering Pub Date : 2025-03-22 DOI:10.1016/j.optlaseng.2025.108954

Zhongquan Wen, Yi Zhou, Shikai Wu, Zhen Li, Kaiyuan Liu, Yurong Li, Jinsheng Zhang, Zhihai Zhang, Jin Xiang, Zhengguo Shang, Gaofeng Liang, Yin She, Gang Chen

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引用次数: 0

Abstract

Owing to its non-invasive nature, high spatial resolution and user-friendly operation, optical microscopy has a wide range of applications. In past decades, tremendous breakthroughs have been made in breaking the diffraction limit in traditional optical imaging. Compared to commonly used fluorescence-based super-resolution microscopy, label-free super-resolution microscopy can extend to the applications unsuitable for fluorescence-labeling, especially for non-organic specimens. In addition, it can avoid the labeling-associated phototoxicity, which frequently occurs during live fluorescence microscopy. However, most reported label-free super-resolution microscopes employed dry lenses with short depth of focus, limiting resolution and complicating lens alignment. Here, we have developed a water-immersion transmission super-resolution optical microscope based on optical needle illumination. Compared to those working in air, the proposed water-immersion transmission super-resolution optical microscope can achieve a lateral resolution of approximately 0.213λ. Such label-free optical super-resolution microscopy is promising for applications where florescence microscopy is not applicable.

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来源期刊

Optics and Lasers in Engineering 工程技术-光学

CiteScore

8.90

自引率

8.70%

发文量

384

审稿时长

42 days

期刊介绍： Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods. Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following: -Optical Metrology- Optical Methods for 3D visualization and virtual engineering- Optical Techniques for Microsystems- Imaging, Microscopy and Adaptive Optics- Computational Imaging- Laser methods in manufacturing- Integrated optical and photonic sensors- Optics and Photonics in Life Science- Hyperspectral and spectroscopic methods- Infrared and Terahertz techniques