Short-day shading increases soluble sugar content in citrus fruit primarily through promoting sucrose distribution, starch degradation and sucrose storage ability

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-14 DOI:10.1016/j.plaphy.2025.109779

Fatima Zaman , Dong-Hai Liu , Yong-Zhong Liu , Muhammad Abbas Khan , Shariq Mahmood Alam , Yin Luo , Han Han , Yan-Ting Li , Abdelmonem Elshahat

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引用次数: 0

Abstract

Sugar is a key factor, significantly affecting fruit flavor quality through sucrose metabolism and accumulation. This study investigated the effect of short-day shading on the accumulation of soluble sugars in citrus fruits by using ‘Nanfeng’ tangerine (Citrus reticulata cv. Nanfeng) as materials. Results revealed that using a black net with 12 % light transmittance for 18-day shading remarkably increased soluble sugars and decreased starch content in fruits. Moreover, expresion levels of two cell wall invertase genes (CwINV6/7) and one sucrose synthase genes (CsSUS6) in the segment membrane (SM) were increased significantly at 18 DAS. On the other hand, in juice sacs (JS), expression levels of vacuolar invertase (VINV), five sucrose synthase genes (CsSUS1-5), two sucrose phosphate synthases (CsSPS1/3), some sugar transporter genes including STP7, SUT1/2/3, two SWEET genes (SWEET5/10), two vacuolar glucose transporter genes (VGT1/2), two tonoplast monosaccharide transporter genes (TMT1/2), and two citrus type I V-PPase genes (CsVPP1/2) were increased significantly at 18DAS as compared to control. Furthermore, starch degradation related gene ISA3, BAM3, GWD and PWD were also significantly induced by 18-day shading. Overall, this study suggested that short-day shading (12 %) enhances soluble sugar accumulation in citrus fruit, primarily by promoting starch degradation and soluble sugar storage in the JS, except for the increase of sucrose distribution to the fruits.

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.