Genome-wide identification of ADF gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis) and functional characterization of BrADF11 under heat stress

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-18 DOI:10.1016/j.plaphy.2025.109796

Wei Wang , Gaoyang Qu , Yifei Sun , Jingyi Chen , Hui Feng , Yue Gao

{"title":"Genome-wide identification of ADF gene family in Chinese cabbage (Brassica rapa L. ssp. pekinensis) and functional characterization of BrADF11 under heat stress","authors":"Wei Wang , Gaoyang Qu , Yifei Sun , Jingyi Chen , Hui Feng , Yue Gao","doi":"10.1016/j.plaphy.2025.109796","DOIUrl":null,"url":null,"abstract":"<div><div>Actin depolymerizing factors (ADFs), which function as essential actin-binding proteins (ABPs), are fundamental to plant growth, development, and stress responses by depolymerizing or severing actin filaments. However, research on the <em>ADF</em> gene family in Chinese cabbage remains relatively scarce. In Chinese cabbage, we identified 18 <em>BrADF</em> genes unevenly distributed across eight chromosomes. Phylogenetic analysis revealed that <em>BrADF</em> genes can be classified into four subfamilies. Cis-regulatory elements related to stress response and hormone signaling response were distributed in the promoter sequences of these genes. Expression analysis showed variability in the expression patterns of <em>BrADF</em> genes across different tissues, with most genes responding to heat stress; notably, <em>BrADF11</em> showed significant upregulation under heat stress conditions. Furthermore, transient silencing and transient overexpression experiments proved that <em>BrADF11</em> plays a negative regulatory role in the heat tolerance of Chinese cabbage. In conclusion, this study not only systematically analyzed the characteristics of <em>ADF</em> gene family in Chinese cabbage, but also laid a solid foundation for studying the function of Chinese Cabbage <em>ADF</em> genes under abiotic stress.</div></div>","PeriodicalId":20234,"journal":{"name":"Plant Physiology and Biochemistry","volume":"223 ","pages":"Article 109796"},"PeriodicalIF":6.1000,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Physiology and Biochemistry","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0981942825003249","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Actin depolymerizing factors (ADFs), which function as essential actin-binding proteins (ABPs), are fundamental to plant growth, development, and stress responses by depolymerizing or severing actin filaments. However, research on the ADF gene family in Chinese cabbage remains relatively scarce. In Chinese cabbage, we identified 18 BrADF genes unevenly distributed across eight chromosomes. Phylogenetic analysis revealed that BrADF genes can be classified into four subfamilies. Cis-regulatory elements related to stress response and hormone signaling response were distributed in the promoter sequences of these genes. Expression analysis showed variability in the expression patterns of BrADF genes across different tissues, with most genes responding to heat stress; notably, BrADF11 showed significant upregulation under heat stress conditions. Furthermore, transient silencing and transient overexpression experiments proved that BrADF11 plays a negative regulatory role in the heat tolerance of Chinese cabbage. In conclusion, this study not only systematically analyzed the characteristics of ADF gene family in Chinese cabbage, but also laid a solid foundation for studying the function of Chinese Cabbage ADF genes under abiotic stress.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.