The identification and expression pattern analysis of carbonic anhydrase gene family in marine medaka (Oryzias melastigma) under low salinity environment

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-09 DOI:10.1016/j.genrep.2025.102187

Binghua Liu , Chang Liu , Lei Lin , Kaiqiang Liu , Yu Yang , Jingjing Zhang , Xiancai Hao , Hong-Yan Wang , Qian Wang , Yuyan Liu , Changwei Shao

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引用次数: 0

Abstract

Salinity difference is a significant characteristic that distinguishes marine and terrestrial aquatic ecosystems. The carbonic anhydrase (CA) plays an important role in responding to the salinity changes in organisms, including regulating ion transport, osmotic balance, and internal environmental homeostasis. However, there is little research on the multiple ca gene family members under salinity stress. Therefore, we analyzed and researched the ca gene family members under different salinity conditions (0 ‰ and 30 ‰) in a euryhaline fish of marine medaka (Oryzias melastigma). In this study, we have screened and identified 18 members of the family in its genome, which are scattered across 10 chromosomes. Although some members had multiple copies, for example, ca16 with 3 copies, there was no homologous pair among the ca genes. These proteins were localized in different cellular compartments (cytoplasm, membrane, mitochondrion, extracellular space, and nucleus) and exhibited α helices, β sheets, and loops, with all containing at least one CA catalytic domain. According to the results of transcriptome and qPCR, we concluded that CA1, CA5A, CA12, CAR12, and CAHZA may respond to low salinity stress. Furthermore, RPF2 may be an important interacting protein with these CA proteins. Our results provide a molecular basis for the salinity domestication of fishes.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.