Tailored microcarriers from solid to porous: Rapid doubling and differentiation behaviors of piscine satellite cells

IF 8.6 2区工程技术 Q1 ENERGY & FUELS

Sustainable Materials and Technologies Pub Date : 2025-03-20 DOI:10.1016/j.susmat.2025.e01368

Qipu Xin , Ruihao Niu , Zhaojing Huang , Jing Yu , Qihe Chen , Donghong Liu , Enbo Xu

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引用次数: 0

Abstract

Microcarriers (MCs) play a crucial role in promoting cells to expand in culture systems for the industries as regenerative medicine products and cell-derived alternative proteins. However, high-performance and biosafe MCs are still urgently needed for cell scale-up expansion under the dynamic shearing environment of bioreactor and pipeline. Here, gelatin was used which is of high biocompatibility and edibility as MC matrix, by TGase-induced crosslinking in combination with emulsification method for piscine satellite cells (PSCs) cultivation. MCs cultivation conditions were optimized in the spinner flasks (6000 MCs/mL, 8:1 ratio of cells to MCs, 50 rpm speed), to achieve about 5 fold of PSCs on Day 9. To further increase the proliferation efficiency, solid MCs were modified to porous MCs through ice templating method, which could lead to ∼6.32 proliferation multiple on Day 9 with high-efficiency differentiation. Also, transcriptome analysis showed that the genes related to cell cycle and DNA replication were obviously upregulated in the MCs groups in comparison to the 2D cultivation group of PSCs. Collectively, these findings demonstrate the ability of porous MCs in realizing large-scale cell expansion and even differentiation.

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来源期刊

Sustainable Materials and Technologies Energy-Renewable Energy, Sustainability and the Environment

CiteScore

13.40

自引率

4.20%

发文量

158

审稿时长

45 days

期刊介绍： Sustainable Materials and Technologies (SM&T), an international, cross-disciplinary, fully open access journal published by Elsevier, focuses on original full-length research articles and reviews. It covers applied or fundamental science of nano-, micro-, meso-, and macro-scale aspects of materials and technologies for sustainable development. SM&T gives special attention to contributions that bridge the knowledge gap between materials and system designs.