Tailored microcarriers from solid to porous: Rapid doubling and differentiation behaviors of piscine satellite cells

IF 8.6 2区工程技术 Q1 ENERGY & FUELS

Sustainable Materials and Technologies Pub Date : 2025-03-20 DOI:10.1016/j.susmat.2025.e01368

Qipu Xin , Ruihao Niu , Zhaojing Huang , Jing Yu , Qihe Chen , Donghong Liu , Enbo Xu

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Abstract

Microcarriers (MCs) play a crucial role in promoting cells to expand in culture systems for the industries as regenerative medicine products and cell-derived alternative proteins. However, high-performance and biosafe MCs are still urgently needed for cell scale-up expansion under the dynamic shearing environment of bioreactor and pipeline. Here, gelatin was used which is of high biocompatibility and edibility as MC matrix, by TGase-induced crosslinking in combination with emulsification method for piscine satellite cells (PSCs) cultivation. MCs cultivation conditions were optimized in the spinner flasks (6000 MCs/mL, 8:1 ratio of cells to MCs, 50 rpm speed), to achieve about 5 fold of PSCs on Day 9. To further increase the proliferation efficiency, solid MCs were modified to porous MCs through ice templating method, which could lead to ∼6.32 proliferation multiple on Day 9 with high-efficiency differentiation. Also, transcriptome analysis showed that the genes related to cell cycle and DNA replication were obviously upregulated in the MCs groups in comparison to the 2D cultivation group of PSCs. Collectively, these findings demonstrate the ability of porous MCs in realizing large-scale cell expansion and even differentiation.

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从固体到多孔的定制微载体：鱼类卫星细胞的快速倍增和分化行为

微载体（MCs）在促进再生医学产品和细胞衍生替代蛋白等行业的培养系统中细胞增殖方面发挥着至关重要的作用。然而，在生物反应器和管道的动态剪切环境下，仍然迫切需要高性能和生物安全的MCs来进行细胞放大扩增。本研究以具有高生物相容性和可食性的明胶为基质，采用tgase诱导交联结合乳化法培养鱼卫星细胞（PSCs）。优化MCs在旋转瓶中的培养条件（6000 MCs/mL，细胞/ MCs比为8:1，转速为50 rpm），第9天可获得约5倍的psc。为了进一步提高增殖效率，通过冰模板法将固体MCs修饰为多孔MCs，在第9天可以实现~ 6.32增殖倍数，并实现高效分化。转录组分析显示，与PSCs 2D培养组相比，MCs组中与细胞周期和DNA复制相关的基因明显上调。总的来说，这些发现证明了多孔MCs实现大规模细胞扩增甚至分化的能力。

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来源期刊

Sustainable Materials and Technologies Energy-Renewable Energy, Sustainability and the Environment

CiteScore

13.40

自引率

4.20%

发文量

158

审稿时长

45 days

期刊介绍： Sustainable Materials and Technologies (SM&T), an international, cross-disciplinary, fully open access journal published by Elsevier, focuses on original full-length research articles and reviews. It covers applied or fundamental science of nano-, micro-, meso-, and macro-scale aspects of materials and technologies for sustainable development. SM&T gives special attention to contributions that bridge the knowledge gap between materials and system designs.