Study on effect of electroporation combining high- and low-frequency harmonics

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-03-17 DOI:10.1016/j.bioelechem.2025.108971

Borja López-Alonso , Tamara Polajžer , Matej Reberšek , Héctor Sarnago , Óscar Lucía , Damijan Miklavčič

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引用次数: 0

Abstract

The effects of electroporation are highly influenced by the shape of the applied waveform. This waveform shape can modify the transmitted energy and current flow patterns, impacting the electric field distribution, temperature rise among others. These interactions, along with their synergies with electroporation, are being explored across various industrial and research domains. For instance, in the biomedical field, high-frequency waveforms such as nanosecond pulses offer distinct advantages, while in the food industry, controlled temperature increases combined with electroporation are beneficial. However, in the medical field, the effects of combining high-frequency waveforms (in the MHz range) with low-frequency waveforms (in the kHz range commonly used in clinical electroporation) have not been thoroughly studied, though hypotheses have been proposed regarding their potential effects.

In this paper, proof of concept of the effect of the combination of two harmonics is presented using three different strategies to investigate new electroporation protocols. To support this study, a specialized electrical and thermal test bench was developed to control and evaluate the feasibility and potential of possible synergy between high- and low-frequency waveforms to electroporation using an in vitro model.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.