An electrochemical biosensor using AuNPs-Ti3C2Tx and ARGET ATRP reactions as signal amplification strategies for ultra-sensitive detection of HER2 protein

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-03-15 DOI:10.1016/j.bioelechem.2025.108970

Yanju Liu , Aozi Feng , Fengzhi Li , Liying Zhao , Qiyong Cai , Daoxiang Li , HuaixiaYang

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Abstract

Human epidermal growth factor receptor 2 (HER2) status is an important factor in evaluating the prognosis of breast cancer patients. Therefore, it is particularly important to develop a simple and sensitive method for the detection of HER2-positive breast cancer. Here, an ultra-sensitive electrochemical biosensor for detecting HER2-specific proteins was assembled using gold nanoparticles and Two-dimensional carbides (AuNPs-Ti₃C₂Tx) as a conducting substrate. The prepared AuNPs-Ti₃C₂Tx not only has good electrical conductivity and strong electrochemical signal output, but also provides a large number of active sites for the AuS bonds assembly aptamer. In addition, the antibodies-modified functionalized graphene oxide (GO) as a carrier platform, which provides an additional boost for the detection of trace targets with high sensitivity under optimal conditions. Afterwards,HER2 protein was detected by signal amplification effect of AuNPs-Ti₃C₂Tx and functionalized GO combined with Electron transfer activated regeneration catalyst atomic transfer radical polymerization (ARGET ATRP). In the range of 1 to 10⁵ ng·mL⁻¹, there was a good linear relationship between the HER2 concentration and the signal intensity, with a limit of detection of 0.19 pg·mL⁻¹. Moreover, this method has good selectivity and stability, and then still maintains good detection performance and strong anti-interference ability in the complex environment of normal human serum, which is expected to be applied in clinical application.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.