Modulation of oxidative stress markers, antioxidant status and inflammatory mediator levels in alloxan-induced diabetic male wistar rats by (Ipomoea batatas) sweet potatoes

Abstract

Introduction

Diabetes mellitus is a metabolic disorder that throws off the equilibrium between reactive oxygen species and antioxidants. To better understand how sweet potato-incorporated feed affects fasting blood glucose levels, antioxidant activity, and the protective benefits of Ipomoea batatas against alloxan-induced diabetic male Wistar rats, this study was conducted.

Methods

Sweet potatoes were analyzed for phytochemicals, proximate compositions, and mineral components. Analysis of the vitamin and amino acid profiles was carried out using High-Performance Liquid Chromatography (HPLC) in accordance with normal procedures. Twenty male rats were randomly selected and divided into four groups. Alloxan was injected intraperitoneally into ten rats at a fasting state, which formed groups B and D (Diabetic control group and Test group), and the remaining ten rats were non-diabetic groups A and C (Non-diabetic control and Test group). The fasting blood glucose levels were measured on days 1, 7 and 14 of the experiment. The rats were sacrificed, and samples were obtained for oxidative stress markers, antioxidant status and inflammatory mediator levels analysis following standard methods.

Results

The findings indicated the presence of vitamin B (cobalamin, biotin, and pantothenic acid), vitamin D (ergosterol, ergocalciferol), and vitamin A (β-carotene, lycopene, retinal, and γ-carotene). The amino acid profile showed the presence of glutamic acid, lysine, tryptophan, glycine, leucine, threonine and cysteine. According to the study, group D had significantly lower fasting blood glucose levels than the control groups, but group B had significantly higher levels than group A (p > 0.05). Oxidative stress activity showed a significant decrease (p > 0.05) in all groups for glutathione peroxidase activity and total antioxidant capacity.

Discussion

The consumption of sweet potatoes has been proven to have anti-diabetic properties and to be therapeutically effective in mellitus and other health issues associated with managing diabetes antioxidant activity.