Techno-functionality of pea protein isolate: Influence of ultrasound and high-pressure homogenization modification methods

IF 8.7 1区化学 Q1 ACOUSTICS

Ultrasonics Sonochemistry Pub Date : 2025-03-23 DOI:10.1016/j.ultsonch.2025.107321

Fereshte Bahmanyar , Mitra Pashaei , Kooshan Nayebzadeh , Ali Dini , Leila Mirmoghtadaie , Hedayat Hosseini

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Abstract

Pea protein isolate (PPI) was modified using high-pressure homogenization (HPH) at three pressure levels (60, 80, and 100 MPa) for three cycles and ultrasound (US) at three power levels (100, 200, and 300 W) for 10 min. Results showed that both techniques significantly increased solubility, oil holding capacity, emulsifying activity and foam capacity. Ultimately, a homogenization pressure of 100 MPa (HPH 100) and an ultrasound power of 300 W (US 300) were identified as the optimal treatments. HPH and US treatments led to a significant reduction in particle size and an increase in the surface charge of the samples. FTIR spectroscopy revealed changes in hydrogen bonding and the secondary structure of PPI after HPH and US treatment. Also, SEM imaging showed that the spherical shape of PPI transformed into heterogeneous sheet structures. Additionally, the comparison of HPH and US techniques revealed that the HPH resulted in a greater reduction in size and a greater increase in solubility and FC compared to the US. On the other hand, the US technique showed greater EAI and thermal stability. Therefore, both HPH and US are effective in altering the PPI structure to enhance its functional properties.

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豌豆分离蛋白的技术功能：超声和高压均质改性方法的影响

采用高压均质（HPH）和超声（US）分别在三种压力水平（60、80和100 MPa）和三种功率水平（100、200和300 W）下对豌豆分离蛋白（PPI）进行改性。结果表明，两种技术均显著提高了豌豆分离蛋白（PPI）的溶解性、持油能力、乳化活性和泡沫容量。最终确定均质压力为100 MPa (HPH 100)，超声功率为300 W （US 300）为最佳处理条件。HPH和US处理显著减小了样品的粒径，增加了样品的表面电荷。FTIR光谱显示了HPH和US处理后PPI的氢键和二级结构的变化。同时，扫描电镜显示，PPI的球形转变为非均质片状结构。此外，HPH和US技术的比较表明，与US相比，HPH的体积减小幅度更大，溶解度和FC增加幅度更大。另一方面，美国技术表现出更高的EAI和热稳定性。因此，HPH和US都能有效地改变PPI的结构，增强其功能性质。

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来源期刊

Ultrasonics Sonochemistry 化学-化学综合

CiteScore

15.80

自引率

11.90%

发文量

361

审稿时长

59 days

期刊介绍： Ultrasonics Sonochemistry stands as a premier international journal dedicated to the publication of high-quality research articles primarily focusing on chemical reactions and reactors induced by ultrasonic waves, known as sonochemistry. Beyond chemical reactions, the journal also welcomes contributions related to cavitation-induced events and processing, including sonoluminescence, and the transformation of materials on chemical, physical, and biological levels. Since its inception in 1994, Ultrasonics Sonochemistry has consistently maintained a top ranking in the "Acoustics" category, reflecting its esteemed reputation in the field. The journal publishes exceptional papers covering various areas of ultrasonics and sonochemistry. Its contributions are highly regarded by both academia and industry stakeholders, demonstrating its relevance and impact in advancing research and innovation.