Isoniazid (INH) a tuberculosis drug detection using turn-off fluorescent [Ce (o-Van)3 (H2O)3] probe in real samples

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-14 DOI:10.1016/j.saa.2025.126057

Rajpal, Subhajit Jana , Ravi Prakash Ojha , Radhe Shyam, Rajiv Prakash

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Abstract

Isoniazid (INH) is an essential antibiotic for the treatment of tuberculosis (TB). The accurate determination of its concentration in the human body is essential and must be monitored carefully because of the risk of severe side effects from prolonged use or overdosing. In this work, we synthesized a turn-off fluorescent sensor based on the coordination complex [Ce(o-Van)₃(H₂O)₃] by a simple precipitation method using cerium (III) nitrate hexahydrate and ortho-vanillin (o-Van) in a 1:3 stoichiometric ratio. The Ce³⁺ ion of the complex is coordinated by three bidentate o-Van ligands and three water molecules. Structural, optical and morphological characterizations were done using HRMS, FT-IR, UV–Vis spectroscopy, XPS, XRD, SEM, and TGA was used to assess thermal stability. The sensor has been proposed to work from the mechanism where the > C=O group of the amide functionality of INH replaces a coordinated water molecule in the complex, leading to fluorescence quenching. With an optimized condition, the fluorescence emission spectra showed the linear response within the concentration ranges 14.47 μM − 147.966 μM with an excellent correlation coefficient value of R² = 0.994. Thus, the proposed sensor had a lower limit of detection (LOD) of 1.63 μM for the INH drug. the as-synthesized [Ce(o-Van)₃(H₂O)₃] probe provides excellent results for the selective detection of INH in pharmaceutical tablets, human urine, and blood samples of healthy people.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.