The biofilm matrix protects Bacillu subtilis against hydrogen peroxide

IF 4.9 Q1 MICROBIOLOGY
Erika Muratov , Julian Keilholz , Ákos T. Kovács , Ralf Moeller
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引用次数: 0

Abstract

Biofilms formed by Bacillus subtilis confer protection against environmental stressors through extracellular polysaccharides (EPS) and sporulation. This study investigates the roles of these biofilm components in resistance to hydrogen peroxide, a common reactive oxygen species source and disinfectant. Using wild-type and mutant strains deficient in EPS or sporulation, biofilm colonies were cultivated at various maturation stages and exposed to hydrogen peroxide. EPS-deficient biofilms exhibited reduced resilience, particularly in early stages, highlighting the structural and protective importance of the matrix. Mature biofilms demonstrated additional protective mechanisms, potentially involving TasA protein fibers and/or the biofilm surface layer (BslA). In contrast, sporulation showed limited contribution to hydrogen peroxide resistance, as survival was primarily matrix-dependent. These findings underscore the necessity of targeting EPS and other matrix components in anti-biofilm strategies, suggesting that hydrogen peroxide-based disinfection could be enhanced by combining it with complementary sporicidal treatments. This study advances our understanding of biofilm resilience, contributing to the development of more effective sterilization protocols.
生物膜基质保护枯草芽孢杆菌免受过氧化氢的侵害
由枯草芽孢杆菌形成的生物膜通过胞外多糖(EPS)和孢子形成,对环境胁迫具有保护作用。本研究探讨了这些生物膜成分在抵抗过氧化氢(一种常见的活性氧源和消毒剂)中的作用。利用缺乏EPS或产孢的野生型和突变型菌株,在不同的成熟阶段培养生物膜菌落并暴露于过氧化氢中。缺乏eps的生物膜表现出较低的弹性,特别是在早期阶段,突出了基质的结构和保护重要性。成熟的生物膜表现出额外的保护机制,可能涉及TasA蛋白纤维和/或生物膜表面层(BslA)。相比之下,孢子形成对过氧化氢抗性的贡献有限,因为存活主要依赖于基质。这些发现强调了在抗生物膜策略中靶向EPS和其他基质成分的必要性,这表明基于过氧化氢的消毒可以通过将其与补充的杀孢剂治疗相结合来增强。这项研究促进了我们对生物膜弹性的理解,有助于开发更有效的灭菌方案。
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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
期刊介绍:
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