TMEM71 is crucial for cell proliferation in lower-grade glioma and is linked to unfavorable prognosis.

IF 5.3 2区 医学 Q1 ONCOLOGY
Jiabao Xie, Wanli Yu, Shikai Gui, Lunshan Peng, Juexian Xiao, Haitao Luo, Zujue Cheng
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引用次数: 0

Abstract

Objective: Transmembrane protein 71 (TMEM71) is implicated in multiple cellular physiological functions and has been demonstrated to be crucial in the advancement of different cancerous growths. However, its specific function in low-grade glioma (LGG) remains unclear.

Methods: We examined the expression patterns and prognostic importance of TMEM71 in various types of cancer by using pan-cancer analysis. The analyses of the correlations between TMEM71 expression and clinicopathological characteristics, prognosis, biological functions, immune characteristics, and genomic variations in LGG were conducted based on its expression patterns. Finally, the expression level and biological function of TMEM71 in LGG were verified by executing in vitro studies.

Results: Abnormal elevation of TMEM71 expression level was associated with poor prognosis of many tumors including LGG. Both multivariate and univariate Cox regression analyses indicated that the expression of TMEM71 served as a standalone prognostic biomarker for LGG. The levels of TMEM71 expression were associated with immune-related characteristics, infiltration of immune cells, immune checkpoint genes (ICPGs) expression, and tumor mutation burden (TMB) in patients with LGG. In laboratory studies, elevated levels of TMEM71 were found to be involved in activating the JAK2/STAT3 pathway, promoting CCAAT/Enhancer Binding Protein D (CEBPD) expression, and ultimately affecting the growth and motility of LGG cells.

Conclusion: TMEM71 is an independent prognostic indicator for LGG and is strongly associated with the growth of LGG cells, positioning it as a potential novel target for treatment.

TMEM71对低级别胶质瘤的细胞增殖至关重要,并与不良预后有关。
目的:跨膜蛋白71 (TMEM71)参与多种细胞生理功能,并已被证明在不同肿瘤生长的进展中起关键作用。然而,其在低级别胶质瘤(LGG)中的具体功能尚不清楚。方法:采用泛癌分析方法检测TMEM71在不同类型肿瘤中的表达模式及其预后意义。基于TMEM71的表达模式,分析其表达与LGG的临床病理特征、预后、生物学功能、免疫特性及基因组变异的相关性。最后,通过体外实验验证TMEM71在LGG中的表达水平和生物学功能。结果:TMEM71表达水平异常升高与包括LGG在内的多种肿瘤预后不良有关。多因素和单因素Cox回归分析表明,TMEM71的表达可作为LGG的独立预后生物标志物。TMEM71表达水平与LGG患者的免疫相关特征、免疫细胞浸润、免疫检查点基因(icpg)表达和肿瘤突变负荷(TMB)相关。在实验室研究中,发现TMEM71水平升高参与激活JAK2/STAT3通路,促进CCAAT/增强子结合蛋白D (CEBPD)表达,最终影响LGG细胞的生长和运动。结论:TMEM71是一种独立的LGG预后指标,与LGG细胞的生长密切相关,是一种潜在的治疗新靶点。
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来源期刊
CiteScore
10.90
自引率
1.70%
发文量
360
审稿时长
1 months
期刊介绍: Cancer Cell International publishes articles on all aspects of cancer cell biology, originating largely from, but not limited to, work using cell culture techniques. The journal focuses on novel cancer studies reporting data from biological experiments performed on cells grown in vitro, in two- or three-dimensional systems, and/or in vivo (animal experiments). These types of experiments have provided crucial data in many fields, from cell proliferation and transformation, to epithelial-mesenchymal interaction, to apoptosis, and host immune response to tumors. Cancer Cell International also considers articles that focus on novel technologies or novel pathways in molecular analysis and on epidemiological studies that may affect patient care, as well as articles reporting translational cancer research studies where in vitro discoveries are bridged to the clinic. As such, the journal is interested in laboratory and animal studies reporting on novel biomarkers of tumor progression and response to therapy and on their applicability to human cancers.
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