Neuroprotective Effect of Rosuvastatin Calcium Combined with Hyperbaric Oxygen Mediated p38MAPK Pathway in Rats with Leukoaraiosis.

IF 1.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cell Biochemistry and Biophysics Pub Date : 2025-03-22 DOI:10.1007/s12013-025-01702-9

Yafeng Shi, Gemin Zhu, Jun Yan, Linxin Zhang, Yongku Du, Zhuoqiong Bian, Jing Fan

{"title":"Neuroprotective Effect of Rosuvastatin Calcium Combined with Hyperbaric Oxygen Mediated p38MAPK Pathway in Rats with Leukoaraiosis.","authors":"Yafeng Shi, Gemin Zhu, Jun Yan, Linxin Zhang, Yongku Du, Zhuoqiong Bian, Jing Fan","doi":"10.1007/s12013-025-01702-9","DOIUrl":null,"url":null,"abstract":"<p><p>This study explored the neuroprotective mechanism of rosuvastatin calcium (RSC) combined with hyperbaric oxygen (HBO) in rats with leukoaraiosis (LA), and its impact on the p38MAPK signaling pathway (SPW). Clean-grade male SD rats were used as subjects, which were assigned into Sham group (SG), LA group (LAG), RSC group (RSCG), HBO group (HBOG), and RSC + HBO group (combination group, CG), 20 rats in each. At 14 d post-modeling, the effects of RSC, HBO, and RSC + HBO treatment on the cognitive function, brain neuronal cell apoptosis, brain tissue matrix metalloproteinases (MMPs) family gene expression, and the status of the p38MAPK SPW in LA rats were analyzed. As against the LAG, the escape latency (EL) was shortened, the count of platform crossings was augmented, the number of brain neuronal cell apoptosis decreased, the relative expression (RE) of brain TIMP-1, MMP-2, MMP-3, and MMP-9 mRNA were reduced (P < 0.05), and the RE of brain cleaved caspase (Cas)-3 and p-p38MAPK proteins were reduced in the RSCG, HBOG, and CG (P < 0.05); As against the RSCG and HBOG, the CG showed more visible improvements in all indicators (P < 0.05). The combination of RSC and HBO can inhibit brain neuronal cell apoptosis and the expression of the MMPs family genes in rats with LA by suppressing the overactivation of the p38MAPK SPW, which is beneficial for the recovery of the rats' cognitive function.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Biochemistry and Biophysics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12013-025-01702-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study explored the neuroprotective mechanism of rosuvastatin calcium (RSC) combined with hyperbaric oxygen (HBO) in rats with leukoaraiosis (LA), and its impact on the p38MAPK signaling pathway (SPW). Clean-grade male SD rats were used as subjects, which were assigned into Sham group (SG), LA group (LAG), RSC group (RSCG), HBO group (HBOG), and RSC + HBO group (combination group, CG), 20 rats in each. At 14 d post-modeling, the effects of RSC, HBO, and RSC + HBO treatment on the cognitive function, brain neuronal cell apoptosis, brain tissue matrix metalloproteinases (MMPs) family gene expression, and the status of the p38MAPK SPW in LA rats were analyzed. As against the LAG, the escape latency (EL) was shortened, the count of platform crossings was augmented, the number of brain neuronal cell apoptosis decreased, the relative expression (RE) of brain TIMP-1, MMP-2, MMP-3, and MMP-9 mRNA were reduced (P < 0.05), and the RE of brain cleaved caspase (Cas)-3 and p-p38MAPK proteins were reduced in the RSCG, HBOG, and CG (P < 0.05); As against the RSCG and HBOG, the CG showed more visible improvements in all indicators (P < 0.05). The combination of RSC and HBO can inhibit brain neuronal cell apoptosis and the expression of the MMPs family genes in rats with LA by suppressing the overactivation of the p38MAPK SPW, which is beneficial for the recovery of the rats' cognitive function.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Cell Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

7.5 months

期刊介绍： Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.