M Karthikeyan, Vishudh Mohan, Purvi Purohit, Vidhu Sharma, Kapil Soni, Bikram Choudhury, Mithu Banerjee, Poonam Elhence, Amit Goyal
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引用次数: 0
Abstract
Purpose: Cholesteatoma is characterized by the abnormal growth of keratinizing squamous epithelium in the middle ear. Although both pediatric and adult populations are affected, differences in the molecular mechanisms underlying cholesteatoma between these age groups are not fully understood. miRNA-21 and IL-6 have been implicated in various inflammatory and proliferative processes, and their roles in cholesteatoma pathogenesis among different age groups warrant detailed investigation. Research objective -Compare the expression levels of miRNA-21 and IL-6 genes in pediatric versus adult cholesteatoma tissues to elucidate potential age-related molecular differences.
Methods: Tissue samples were collected from 30 patients (15 in Pediatric group and 15 in Adult group) undergoing surgical treatment for cholesteatoma. Preoperative MERI scores and Quantitative real-time PCR (qRT-PCR) was employed to measure the expression levels of miRNA-21 and IL-6. Statistical analyses were conducted to compare the expression profiles between the two groups.
Results: The results revealed a significantly higher expression of miRNA-21 and IL-6 in pediatric cholesteatoma compared to adult cholesteatoma. These findings suggest distinct molecular pathways may be involved in the pathogenesis of cholesteatoma in different age groups.
Conclusion: The differential expression of miRNA-21 and IL-6 between pediatric and adult cholesteatoma underscores the potential for age-specific therapeutic targets. Increased miRNA-21 and IL-6 gene expression in pediatric cases may indicate a more pronounced role in tissue proliferation and inflammation. Further research is necessary to explore the clinical implications and potential for targeted treatments based on these molecular differences.
期刊介绍：
Official Journal of
European Union of Medical Specialists – ORL Section and Board
Official Journal of Confederation of European Oto-Rhino-Laryngology Head and Neck Surgery
"European Archives of Oto-Rhino-Laryngology" publishes original clinical reports and clinically relevant experimental studies, as well as short communications presenting new results of special interest. With peer review by a respected international editorial board and prompt English-language publication, the journal provides rapid dissemination of information by authors from around the world. This particular feature makes it the journal of choice for readers who want to be informed about the continuing state of the art concerning basic sciences and the diagnosis and management of diseases of the head and neck on an international level.
European Archives of Oto-Rhino-Laryngology was founded in 1864 as "Archiv für Ohrenheilkunde" by A. von Tröltsch, A. Politzer and H. Schwartze.
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