Denatonium benzoate promotes IgE-mediated mast cell degranulation and ovalbumin-induced allergic diarrhea in BALB/c mice.

IF 2.3 4区 医学 Q3 ALLERGY
Ju Liu, Huaping Xu, Shaotong Feng, Xiaoyun Shi, Xin Li, Jiexin Zou, Linbo Shi
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引用次数: 0

Abstract

Background: Denatonium benzoate (DB), one of the most bitter compounds known to man, is used for alcohol denaturation. Some reports have demonstrated that asthmatic symptoms are associated with DB exposure, but the possible links between DB and immunoglobulin E (IgE)-mediated allergy susceptibility have not yet been examined.

Objective: This study investigated the effects of DB on in vitro IgE-mediated mast cell degranulation and food allergy in BALB/c mice.

Methods: IgE-sensitized rat RBL-2H3 cells (a basophilic leukemia mast cell line) and human KU812 cells (a basophilic cell line) and mice with ovalbumin (OVA)-induced allergy were treated with DB. Histamine release, calcium ion (Ca²⁺) influx, phosphorylated spleen tyrosine kinase (p-Lyn) and phosphorylated phospholipase C-γ (p-PLCγ) levels, OVA-specific IgE, anaphylactic symptoms, and the cell-surface expression of the high-affinity IgE receptor α-subunit (FcεRIα) on mast cells were evaluated.

Results: DB increased histamine release, Ca²⁺ mobilization, and p-Lyn and p-PLCγ levels in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα messenger RNA (mRNA). In mice, DB increased the severity of OVA-induced anaphylactic and diarrheic symptoms, the mucus thickness in the jejunum, histamine and OVA-specific IgE levels, and FcεRIα mRNA in isolated mucosal mast cells.

Conclusions: Our work indicates that DB promotes IgE-mediated mast cell degranulation and OVA-induced allergic diarrhea in BALB/c mice, providing evidence that exposure to DB promotes allergy susceptibility.

苯甲酸地那铵促进BALB/c小鼠ige介导的肥大细胞脱颗粒和卵清蛋白诱导的过敏性腹泻。
背景:苯甲酸地那铵(DB)是人类已知的最苦的化合物之一,用于酒精变性。一些报告表明,哮喘症状与DB暴露有关,但DB与免疫球蛋白E (IgE)介导的过敏易感性之间的可能联系尚未得到检验。目的:研究DB对体外ige介导的BALB/c小鼠肥大细胞脱颗粒和食物过敏的影响。方法:对ige致敏的大鼠RBL-2H3细胞(一种嗜碱性白血病肥大细胞系)和人KU812细胞(一种嗜碱性白血病细胞系)以及卵清蛋白(OVA)致过敏小鼠进行DB治疗。评估组胺释放、钙离子(Ca 2 +)内流、磷酸化脾酪氨酸激酶(p-Lyn)和磷酸化磷脂酶C-γ (p-PLCγ)水平、ova特异性IgE、过敏症状和高亲和力IgE受体α-亚基(FcεRIα)在肥大细胞表面的表达。结果:DB增加了ige介导的活化RBL-2H3和KU812细胞中组胺的释放、Ca 2 +的动员以及p-Lyn和p-PLCγ水平,增强了细胞表面FcεRIα信使RNA (mRNA)的表达。在小鼠中,DB增加ova诱导的过敏和腹泻症状的严重程度,空肠粘液厚度,组胺和ova特异性IgE水平,以及离体粘膜肥大细胞中FcεRIα mRNA。结论:我们的研究表明,DB促进ige介导的肥大细胞脱颗粒和ova诱导的BALB/c小鼠过敏性腹泻,为暴露于DB促进过敏易感性提供了证据。
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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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