Analysis of daily aspirin intake on platelet-associated factors and aggregation in nonsteroidal anti-inflammatory drug exacerbated respiratory disease: A cross-sectional study.

IF 2.3 4区 医学 Q3 ALLERGY
Selcan Genc, Basak Ezgi Sarac, Ozge Can Bostan, Gulseren Tuncay, Hayriye Akel Bilgic, Baran Erman, Umit Sahiner, Gul Karakaya, Ali Fuat Kalyoncu, Ebru Damadoglu, Cagatay Karaaslan
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引用次数: 0

Abstract

Background: Nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD) is a clinical syndrome characterized by chronic rhinosinusitis with nasal polyposis (CRSwNP), adult-onset asthma and hypersensitivity to NSAIDs. Long-term aspirin treatment after desensitization (ATAD) is used for clinical improvement in N-ERD patients. However, information on the potential effect of ATAD on the platelet-neutrophil aggregates (PNA) level in N-ERD patients is highly limited.

Objective: This study aimed to explore the impact of PNA on the pathogenesis of N-ERD and the potential effect of ATAD on N-ERD patient profiles from a platelet point-of-view.

Methods: Sixty-one individuals were enrolled, including 16 N-ERD patients with ATAD (ATAD+), 15 N-ERD patients without ATAD (ATAD-), 15 aspirin-tolerant asthma (ATA) patients, and 15 healthy controls (HCs). Lipid mediators classical in N-ERD, including urinary-LTE4 (uLTE4), prostaglandin-D2 (PGD2), and prostaglandin-E2 (PGE2) were assessed by ELISA. Platelet activation was estimated based on expression levels of sP-selectin, CD40L, Platelet Factor-4 (PF4), RANTES, Thromboxane-A2 (TXA2), PAF, 12-HETE in plasma levels by ELISA; and PNA percentage by flow cytometry.

Results: ATAD+; 12-HETE, and PF4 levels were remarkably low, while higher levels were determined in ATAD- and ATA groups. ATAD+; uLTE4 levels were positively correlated with 12-HETE. Another positive correlation was detected between sP-selectin and 12-HETE in ATAD-. Compared to HCs, it was found that among all N-ERD patients, significant increase in PNA.

Conclusions: Plasma levels of PGE2, PF4, and 12-HETE appear to be affected by aspirin treatment. We believe that 12-HETE could play a significant role in the N-ERD pathogenesis by contributing to platelet activation.

每日阿司匹林摄入量对血小板相关因子和非甾体抗炎药聚集性加重呼吸系统疾病的影响分析:一项横断面研究
背景:非甾体抗炎药(NSAID)加重呼吸系统疾病(N-ERD)是一种以慢性鼻窦炎伴鼻息肉病(CRSwNP)、成人哮喘和对非甾体抗炎药过敏为特征的临床综合征。脱敏后长期阿司匹林治疗(ATAD)用于N-ERD患者的临床改善。然而,关于ATAD对N-ERD患者血小板-中性粒细胞聚集物(PNA)水平的潜在影响的信息非常有限。目的:本研究旨在从血小板的角度探讨PNA对N-ERD发病机制的影响以及ATAD对N-ERD患者谱的潜在影响。方法:纳入61例患者,包括16例伴有ATAD的N-ERD患者(ATAD+)、15例无ATAD的N-ERD患者(ATAD-)、15例阿司匹林耐受性哮喘患者(ATA)和15例健康对照(hc)。采用ELISA法检测N-ERD典型的脂质介质,包括尿lte4 (uLTE4)、前列腺素d2 (PGD2)和前列腺素e2 (PGE2)。ELISA法检测sp -选择素、CD40L、血小板因子-4 (PF4)、RANTES、血栓素- a2 (TXA2)、PAF、12-HETE在血浆中的表达水平;流式细胞术检测PNA百分比。结果:ATAD +;12-HETE和PF4水平明显较低,而ATAD和ATA组的水平较高。ATAD +;uLTE4水平与12-HETE呈正相关。sp -选择素与ATAD-中的12-HETE也存在正相关。与hc相比,所有N-ERD患者的PNA均显著升高。结论:血浆PGE2、PF4和12-HETE水平似乎受到阿司匹林治疗的影响。我们认为12-HETE可能通过促进血小板活化在N-ERD发病机制中发挥重要作用。
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来源期刊
CiteScore
12.80
自引率
0.00%
发文量
74
审稿时长
>12 weeks
期刊介绍: The Asian Pacific Journal of Allergy and Immunology (APJAI) is an online open access journal with the recent impact factor (2018) 1.747 APJAI published 4 times per annum (March, June, September, December). Four issues constitute one volume. APJAI publishes original research articles of basic science, clinical science and reviews on various aspects of allergy and immunology. This journal is an official journal of and published by the Allergy, Asthma and Immunology Association, Thailand. The scopes include mechanism, pathogenesis, host-pathogen interaction, host-environment interaction, allergic diseases, immune-mediated diseases, epidemiology, diagnosis, treatment and prevention, immunotherapy, and vaccine. All papers are published in English and are refereed to international standards.
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