Establishment of GDF15 time-resolved fluorescence immunoassay and its clinical application in colorectal cancer

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2025-03-19 DOI:10.1016/j.ab.2025.115848

Meichun Chen , Hongming Fang , Shang Gao , Tianyu Zheng , Shangbin Kao , Yuan Qin , Xueqin Zhao , Xiumei Zhou , Bao Zhu , Biao Huang

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引用次数: 0

Abstract

Objective

This study aimed to develop a highly sensitive time-resolved fluoroimmunoassay for growth differentiation factor 15 (GDF15-TRFIA) and investigate its clinical applicability in colorectal cancer (CRC).

Methods

Using the principle of double-antibody sandwich immunity, the GDF15-TRFIA was established by solid-phase capture antibody and labeled detection antibody with europium as a tracer, the levels of serum GDF15 were quantified in healthy controls (HCs) and patients, and the value of GDF15 in the diagnosis of CRC was analyzed.

Results

The established method has a wide measurement range and good linearity. The HOOK effect was not observed when GDF15 was less than 2000 ng/mL. The intra-analytical coefficients of variation (CVs) were 3.27 %–4.54 %, and the inter-analytical CVs were 5.84 %–10.41 %, and recoveries were 88.15 %–112.36 %. The correlation between GDF15-TRFIA and ELISA was good (ρ = 0.9284). Serum GDF15 levels were significantly higher in CRC patients than in benign colorectal tumor (BCT) patients and HCs (P < 0.0001). ROC analysis showed that simultaneous detection of CEA, CA19-9, and GDF15 significantly improved the diagnostic efficiency of CEA and CA19-9.

Conclusion

A highly sensitive GDF15-TRFIA method for serum GDF15 was successfully established. It can be used for preliminary diagnosis of CRC, and expected to be a good auxiliary tool for the future clinical diagnosis of CRC.

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.