Antifungal resistance in yeasts from One Health perspective: A Brazilian study

IF 8.2 1区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Science of the Total Environment Pub Date : 2025-03-20 DOI:10.1016/j.scitotenv.2025.179139

Rafael Wesley Bastos , Nalu Teixeira de Aguiar Peres , Kássia Jéssica Galdino da Silva , Ludmila Gouveia Eufrasio , Daniel Santana de Carvalho , Gabriela Silva Cruz , Fabíola Lucini , Lorena Souza Silva , Sthefany Emanuelle Silva , Hareton Teixeira Vechi , Manoella do Monte Alves , Luciana Rodrigues Ferreira Ribeiro , Karine Lilian de Souza , José Aparecido Moreira , Janete Gouveia de Souza , Isabela Lima de Miranda , Gustavo Jose Cota de Freitas , Victor Augusto Teixeira Leocádio , Danielle Leticia da Silva , Lislaina dos Santos Corrêa , Daniel Assis Santos

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Abstract

Environmental exposure to fungicides can induce cross-resistance in filamentous fungi, but little is known about their effects on medically important yeasts. This multicenter study investigated the resistance in Candida isolated from different sources and evaluated the influence of clinical and environmental antifungals. Different yeasts were collected from patients, hospital environments, swine, poultry and their surroundings in four different states of Brazil. A total of 571 isolates were identified, of which 82.5 % belonged to the genus Candida. Antifungal susceptibility testing revealed that C. tropicalis had the highest resistance rate (12 %) to fluconazole, followed by C. glabrata and C. albicans with resistance rates of 8 % and 6 %, respectively. Resistance to micafungin was most common in C. glabrata (15 %). For amphotericin B, we identified two non-wild-type isolates, one belonging to the C. tropicalis species and the other to C. glabrata. Most resistant isolates were recovered from patients; however, resistance was also observed in animal and environmental isolates. In addition, susceptibility to tebuconazole, an environmental azole, was evaluated and a cut-off value of 8 μg/mL was proposed to assess potential resistance. Among the isolates, 10.5 % had a high minimum inhibitory concentrations (MIC) for tebuconazole. Moreover, cross-resistance is possible to occur, as 3.6 % of Candida isolates were resistant to fluconazole and had high MIC for tebuconazole, particularly C. albicans, C. tropicalis, and C. krusei. This study demonstrates the importance of monitoring yeast resistance to antifungals and environmental fungicides in different niches, and calls for a multidisciplinary approach to understand multiple factors involved in resistance development.

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来源期刊

Science of the Total Environment 环境科学-环境科学

CiteScore

17.60

自引率

10.20%

发文量

8726

审稿时长

2.4 months

期刊介绍： The Science of the Total Environment is an international journal dedicated to scientific research on the environment and its interaction with humanity. It covers a wide range of disciplines and seeks to publish innovative, hypothesis-driven, and impactful research that explores the entire environment, including the atmosphere, lithosphere, hydrosphere, biosphere, and anthroposphere. The journal's updated Aims & Scope emphasizes the importance of interdisciplinary environmental research with broad impact. Priority is given to studies that advance fundamental understanding and explore the interconnectedness of multiple environmental spheres. Field studies are preferred, while laboratory experiments must demonstrate significant methodological advancements or mechanistic insights with direct relevance to the environment.