Specific capture, detection, and killing of Enterococcus faecalis based on aptamer-modified peroxidase mimetic nanozymes

IF 13.2 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Xiangjun Yang, Yi Hu, Haonan Zhou, Na Lu, Min Zhang, Zisheng Tang
{"title":"Specific capture, detection, and killing of Enterococcus faecalis based on aptamer-modified peroxidase mimetic nanozymes","authors":"Xiangjun Yang, Yi Hu, Haonan Zhou, Na Lu, Min Zhang, Zisheng Tang","doi":"10.1016/j.cej.2025.161848","DOIUrl":null,"url":null,"abstract":"Pathogenic bacteria in the human oral cavity are the leading causes of oral disease, like dental caries and root canal infections. Conventional broad-spectrum oral sterilization easily kills most bacteria indiscriminately, and may destroy microbial community balance and cause oral biofilm-related diseases. However, a platform that enables all-in-one specific capture, detecting, and killing for one type of bacteria rather than targeting other bacteria is desirable. Here, we demonstrate that a DNA aptamer-modified hierarchical magnetic copper silicate decorated Fe<sub>3</sub>O<sub>4</sub> nanozyme conjugate (Apt-CuSi@Fe) can efficiently mediate selective recognition, detection, and rapid killing of oral pathogen <em>Enterococcus faecalis</em> (<em>E. faecalis</em>). Needle-like magnetic nanostructures with peroxidase (POD)-like activity were synthesized by a simple hydrothermal method, and then DNA aptamers were functionalized on their surface to achieve magnetic targeted separation and visual detection of <em>E. faecalis</em>. Moreover, the efficient reactive oxygen species (ROS) generation ability and unique hierarchical structure of Apt-CuSi@Fe conjugates allowed for selective bacterial killing. At a concentration of 50 μg/mL, the sterilization efficiency of aptamer-functionalized nanozyme probes against <em>E. faecalis</em> exceeded 98 %, far superior to that of bare CuSi@Fe nanozymes (61.3 %). In addition, it effectively inhibited the formation of <em>E. faecalis</em> biofilms. The synergetic antibacterial mechanism combines physical damage to the bacterial membrane via piercing puncture with the efficient diffusion and binding of target-enhanced ROS, thereby promoting such a prompted antibacterial activity. The proposed all-in-one platform integrates precise capture, sensitive detection, and targeted killing of <em>E. faecalis</em>, with potential applications in the integrated diagnosis and treatment of oral infectious diseases and targeted eradication of bacterial infections.","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":"10 1","pages":""},"PeriodicalIF":13.2000,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.cej.2025.161848","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Pathogenic bacteria in the human oral cavity are the leading causes of oral disease, like dental caries and root canal infections. Conventional broad-spectrum oral sterilization easily kills most bacteria indiscriminately, and may destroy microbial community balance and cause oral biofilm-related diseases. However, a platform that enables all-in-one specific capture, detecting, and killing for one type of bacteria rather than targeting other bacteria is desirable. Here, we demonstrate that a DNA aptamer-modified hierarchical magnetic copper silicate decorated Fe3O4 nanozyme conjugate (Apt-CuSi@Fe) can efficiently mediate selective recognition, detection, and rapid killing of oral pathogen Enterococcus faecalis (E. faecalis). Needle-like magnetic nanostructures with peroxidase (POD)-like activity were synthesized by a simple hydrothermal method, and then DNA aptamers were functionalized on their surface to achieve magnetic targeted separation and visual detection of E. faecalis. Moreover, the efficient reactive oxygen species (ROS) generation ability and unique hierarchical structure of Apt-CuSi@Fe conjugates allowed for selective bacterial killing. At a concentration of 50 μg/mL, the sterilization efficiency of aptamer-functionalized nanozyme probes against E. faecalis exceeded 98 %, far superior to that of bare CuSi@Fe nanozymes (61.3 %). In addition, it effectively inhibited the formation of E. faecalis biofilms. The synergetic antibacterial mechanism combines physical damage to the bacterial membrane via piercing puncture with the efficient diffusion and binding of target-enhanced ROS, thereby promoting such a prompted antibacterial activity. The proposed all-in-one platform integrates precise capture, sensitive detection, and targeted killing of E. faecalis, with potential applications in the integrated diagnosis and treatment of oral infectious diseases and targeted eradication of bacterial infections.
基于拟合物修饰过氧化物酶模拟纳米酶的粪肠球菌特异性捕获、检测和杀灭技术
人类口腔中的致病菌是导致龋齿和根管感染等口腔疾病的主要原因。传统的广谱口腔消毒容易无差别地杀死大部分细菌,破坏微生物群落平衡,引起口腔生物膜相关疾病。然而,一种能够对一种细菌而不是针对其他细菌进行特异性捕获、检测和杀死的平台是理想的。在这里,我们证明了DNA适体修饰的分层磁性硅酸铜修饰的Fe3O4纳米酶偶联物(Apt-CuSi@Fe)可以有效地介导口腔病原体粪肠球菌(E. faecalis)的选择性识别、检测和快速杀死。采用简单的水热法合成了具有过氧化物酶(POD)样活性的针状磁性纳米结构,并在其表面功能化DNA适配体,实现了粪肠杆菌的磁性靶向分离和视觉检测。此外,Apt-CuSi@Fe缀合物高效的活性氧(ROS)生成能力和独特的层次结构允许选择性杀死细菌。在50 μg/mL浓度下,适体功能化纳米酶探针对粪肠杆菌的杀菌效率超过98 %,远优于单纯的CuSi@Fe纳米酶(61.3 %)。此外,它还能有效抑制粪肠球菌生物膜的形成。其协同抗菌机制将刺穿对细菌膜的物理损伤与靶向增强ROS的有效扩散和结合结合在一起,从而促进了这种促进的抗菌活性。该平台集粪肠杆菌的精准捕获、灵敏检测和靶向杀灭于一体,在口腔感染性疾病的综合诊疗和细菌感染的靶向根除方面具有潜在的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Chemical Engineering Journal
Chemical Engineering Journal 工程技术-工程:化工
CiteScore
21.70
自引率
9.30%
发文量
6781
审稿时长
2.4 months
期刊介绍: The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信