[Screening and Identification of Polyvinyl Chloride-degrading Bacteria and Its Degradation Characteristics].

Q2 Environmental Science
Shuai-Bing Wang, Wei Li, Dan-Dan Wang, Chao Wang, Xiang-Hui Yan, Na Geng, Zhi-Guo Zhao, Zhi-Hong Xie
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引用次数: 0

Abstract

Polyvinyl chloride (PVC) has been shown to be degraded by some insect larvae; however, research is scarce on degrading bacteria. This study revealed that Zophobas atratus larvae were able to keep a higher survival rate only when taking in PVC and reached up to 72.33% within 30 d. Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) showed that the peak values of the major functional groups of PVC in the frass changed significantly, and the thermal stability decreased, indicating that PVC biodegraded in the gut of Z. atratus. Based on these, three PVC-degrading bacteria were isolated from the gut contents of the larvae with PVC as the only carbon source in the culture medium for selection, which were identified to be Enterococcus faecalis, Enterobacter hormaechei, and Bacillus subtilis. The degradation test of PVC showed that C5-1 had the greatest degradation efficiency among the three strains, which resulted in the weight loss of (6.19 ± 0.14) mg, and the water contact angle (WCA) decreased by 9.4° within 50 d, effectively improving the hydrophilicity of PVC. In conclusion, the strain C5-1 isolated from the gut of Z. atratus larvae showed the high potential to be applied to the biodegradation of PVC plastics.

聚氯乙烯降解菌的筛选鉴定及其降解特性
聚氯乙烯(PVC)已被证明可以被一些昆虫幼虫降解,但对降解细菌的研究很少。本研究发现,长尾溞幼虫只有在摄入PVC时才能保持较高的存活率,在30 d内可达到72.33%。傅里叶红外光谱(FTIR)和热重分析(TGA)表明,草中PVC主要官能团的峰值发生了显著变化,热稳定性下降,表明PVC在长尾溞肠道内发生了生物降解。在此基础上,以聚氯乙烯为唯一碳源进行培养基筛选,从幼虫肠道内容物中分离出3种降解聚氯乙烯的细菌,鉴定为粪肠球菌(Enterococcus faecalis)、荷氏肠杆菌(Enterobacter hormaechei)和枯草芽孢杆菌。对PVC的降解试验表明,C5-1在3株菌株中降解效率最高,在50 d内失重(6.19±0.14)mg,水接触角(WCA)降低9.4°,有效提高了PVC的亲水性。综上所述,从白腹弧菌幼虫肠道中分离得到的菌株C5-1在生物降解PVC塑料方面具有很高的应用潜力。
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来源期刊
环境科学
环境科学 Environmental Science-Environmental Science (all)
CiteScore
4.40
自引率
0.00%
发文量
15329
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