Changping Jiang, Yanyan Du, Zhongyu Lou, Yanping Zhang, Tai Wang
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引用次数: 0
Abstract
Rhinogobio nasutus is an endemic fish species native to the middle and upper reaches of the Yellow River in China, renowned for its high economic and nutritional value. However, due to overfishing, human activities, and environmental pollution, it is now critically endangered. This study presents the construction of a telomere-to-telomere (T2T) reference genome for R. nasutus by integrating PacBio HiFi reads, Oxford Nanopore Technologies and Hi-C data. The assembled genome has a total size of 953.38 Mb and is anchored to 25 autosomes. Multiple assessments confirmed the high quality of the genome in terms of mapping rate (99.77% and 99.85%), completeness (BUSCO: 99.31%), and accuracy (QV: 47.05 and 53.10). Functional annotation was achieved for 98.16% of the 24,677 protein-coding genes, with a BUSCO score of 99.81%. This work not only facilitates the genetic conservation of R. nasutus but also provides a valuable resource for related evolutionary studies.
期刊介绍：
Scientific Data is an open-access journal focused on data, publishing descriptions of research datasets and articles on data sharing across natural sciences, medicine, engineering, and social sciences. Its goal is to enhance the sharing and reuse of scientific data, encourage broader data sharing, and acknowledge those who share their data.
The journal primarily publishes Data Descriptors, which offer detailed descriptions of research datasets, including data collection methods and technical analyses validating data quality. These descriptors aim to facilitate data reuse rather than testing hypotheses or presenting new interpretations, methods, or in-depth analyses.
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