Seminal plasma PGK2 serves as a predictive biomarker for post-varicocelectomy sperm motility improvement in varicocele subjects.

IF 1.9 3区 医学 Q4 ANDROLOGY
Translational andrology and urology Pub Date : 2025-02-28 Epub Date: 2025-02-25 DOI:10.21037/tau-24-629
Cheng Yang, Jianxiong Li, Shenghao Wu, Cundong Liu, Ranran Zhou, Shan-Chao Zhao
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引用次数: 0

Abstract

Background: Not all varicocele (VC) patients experience improvements in semen quality after undergoing varicocelectomy. This study aims to investigate whether phosphoglycerate kinase 2 (PGK2) protein in seminal plasma could serve as a predictive biomarker for the enhancement of sperm motility post-varicocelectomy.

Methods: Sixty patients diagnosed with VC-associated asthenospermia and who underwent varicocelectomy at the Third Affiliated Hospital of Southern Medical University were included in this study. Semen samples from 33 healthy donors were also collected. Hematological and semen analysis parameters were assessed at baseline and at the 6-month postoperative follow-up. The concentration of PGK2 in seminal plasma was determined using enzyme-linked immunosorbent assay (ELISA). Additionally, eleven machine learning algorithms were employed to develop a model to evaluate the effectiveness of varicocelectomy based on PGK2 concentration, hematological parameters, and semen quality indicators.

Results: The levels of PGK2 in seminal plasma were significantly lower in VC patients compared to healthy donors (P<0.001). Increased levels of PGK2 were observed in the VC subjects after the varicocelectomy (P<0.001). PGK2 concentration was found to be a significant indicator [area under curve (AUC) =0.735, 95% confidence interval (CI): 0.601-0.860] and an independent predictor (P=0.003, P=0.049) of the success of varicocelectomy. The linear discriminant analysis (LDA) model demonstrated strong classification ability for assessing varicocelectomy outcomes in both the training (AUC =1.000, 95% CI: 1.000-1.000) and testing (AUC =0.804, 95% CI: 0.619-0.963) groups.

Conclusions: PGK2 in seminal plasma shows promise as a potential biomarker for predicting improved sperm motility following varicocelectomy, thus aiding in clinical decision-making regarding therapeutic approaches.

背景:并非所有精索静脉曲张(VC)患者在接受精索静脉曲张切除术后精液质量都会得到改善。本研究旨在探讨精浆中的磷酸甘油酸激酶 2(PGK2)蛋白是否可作为精索静脉曲张切除术后提高精子活力的预测性生物标志物:本研究纳入了在南方医科大学第三附属医院接受精索静脉曲张切除术的60例VC相关性少精症患者。研究还收集了 33 名健康供体的精液样本。在基线和术后 6 个月随访时对血液学和精液分析参数进行了评估。精浆中 PGK2 的浓度是通过酶联免疫吸附试验(ELISA)测定的。此外,还采用了11种机器学习算法来建立一个模型,根据PGK2浓度、血液学参数和精液质量指标来评估精索静脉曲张切除术的效果:结果:与健康捐献者相比,精索静脉曲张患者精浆中的 PGK2 含量明显较低(结论:精索静脉曲张患者精浆中的 PGK2 含量明显高于健康捐献者):精浆中的PGK2有望成为预测精索静脉曲张切除术后精子活力改善情况的潜在生物标志物,从而有助于临床治疗方法的决策。
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来源期刊
CiteScore
4.10
自引率
5.00%
发文量
80
期刊介绍: ranslational Andrology and Urology (Print ISSN 2223-4683; Online ISSN 2223-4691; Transl Androl Urol; TAU) is an open access, peer-reviewed, bi-monthly journal (quarterly published from Mar.2012 - Dec. 2014). The main focus of the journal is to describe new findings in the field of translational research of Andrology and Urology, provides current and practical information on basic research and clinical investigations of Andrology and Urology. Specific areas of interest include, but not limited to, molecular study, pathology, biology and technical advances related to andrology and urology. Topics cover range from evaluation, prevention, diagnosis, therapy, prognosis, rehabilitation and future challenges to urology and andrology. Contributions pertinent to urology and andrology are also included from related fields such as public health, basic sciences, education, sociology, and nursing.
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