Profile of cocaine and amphetamine regulated transcript peptide (CARTp) and Corticotropin-releasing factor (CRF) in the brain of Euphlyctis cyanophlyctis during puddle desiccation

IF 2.5 3区医学 Q3 ENDOCRINOLOGY & METABOLISM

Neuropeptides Pub Date : 2025-03-15 DOI:10.1016/j.npep.2025.102518

Swapnil A. Shewale , Sunil J. Koli , Sneha Sagarkar , Ameeta Ravikumar , Shobha Bhargava

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引用次数: 0

Abstract

During the post breeding season, anuran tadpoles are subjected to a wide range of environmental variables due to intermittent dry spells leading to pond desiccation. To avoid these harsh conditions, anurans accelerate rate of metamorphosis. In anurans, hypothalamic corticotropin-releasing factor (CRF) senses stress and activates metamorphosis via hypothamalo-pituitary- interenal/thyroid axis. Cocaine and amphetamine regulated transcript peptide (CARTp) is widely reported in the brains of vertebrates except reptiles. Role of CARTp along with CRF in mitigating the physiological effects of stress in mammals is well established. Therefore, we investigated effect of puddle desiccation on the CARTp and CRF peptide immunoreactivity in the brain of prometamorphic tadpoles of Euphlyctis cyanophlyctis. Animals in the naturally occurring desiccating puddle were fixed on field and the expression of CARTp and CRF were studied using immunohistochemistry. CARTp-immunoreactivity in entopeduncular nucleus (AEN and PEN), nucleus medialis septi (NMS) and hypothalamus (dHy and vHy) significantly increased in the prometamorphic tadpoles exposed to puddle desiccation as compared to controls. In preoptic area (POA) and Edinger Westphal nucleus (EW) significant increase in CARTp as well as CRF immunoreactive cells were observed in puddle desiccation group. Strong CRF immunoreactivity was observed in median eminence of stressed group. Puddle desiccation induced increase in the expression of CARTp and CRF peptide in the neuroendocrine axis may suggest their involvement in environmental stress induced accelerated metamorphosis. Moreover, elevated expression of CARTp but not CRF peptide in regions regulating energy homeostasis may indicate starvation due to scarcity of food or stress induced appetite suppression.

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可卡因和安非他明在水坑干燥时调节蓝绿炎脑内转录肽（CARTp）和促肾上腺皮质激素释放因子（CRF）的特征。

在繁殖后的季节，由于间歇性干旱导致池塘干燥，无尾蝌蚪受到各种环境变量的影响。为了避免这些恶劣的环境，无脊椎动物加速了变态的速度。在无尾动物中，下丘脑促肾上腺皮质激素释放因子（CRF）通过下丘脑-垂体-内/甲状腺轴感知压力并激活变态。除爬行动物外，可卡因和安非他命调节转录肽（CARTp）广泛存在于脊椎动物的大脑中。CARTp和CRF在减轻哺乳动物应激生理效应中的作用已得到证实。因此，我们研究了水坑脱水对青绿幼稚蚪脑内CARTp和CRF肽免疫反应性的影响。将自然发生的干燥水坑中的动物固定在野外，用免疫组织化学方法研究CARTp和CRF的表达。与对照组相比，暴露于水坑脱水的早变质蝌蚪核内核（AEN和PEN）、中隔核（NMS）和下丘脑（dHy和vHy）的cartp免疫反应性显著升高。水坑干燥组视前区（POA）和Edinger Westphal核（EW）中CARTp和CRF免疫反应细胞显著升高。应激组正中隆起可见较强的CRF免疫反应性。水坑脱水导致神经内分泌轴上CARTp和CRF肽表达增加，可能与环境应激诱导的加速变态有关。此外，在调节能量稳态的区域，CARTp而非CRF肽的表达升高可能表明由于食物缺乏或应激引起的食欲抑制而导致饥饿。

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来源期刊

Neuropeptides 医学-内分泌学与代谢

CiteScore

5.40

自引率

6.90%

发文量

审稿时长

>12 weeks

期刊介绍： The aim of Neuropeptides is the rapid publication of original research and review articles, dealing with the structure, distribution, actions and functions of peptides in the central and peripheral nervous systems. The explosion of research activity in this field has led to the identification of numerous naturally occurring endogenous peptides which act as neurotransmitters, neuromodulators, or trophic factors, to mediate nervous system functions. Increasing numbers of non-peptide ligands of neuropeptide receptors have been developed, which act as agonists or antagonists in peptidergic systems. The journal provides a unique opportunity of integrating the many disciplines involved in all neuropeptide research. The journal publishes articles on all aspects of the neuropeptide field, with particular emphasis on gene regulation of peptide expression, peptide receptor subtypes, transgenic and knockout mice with mutations in genes for neuropeptides and peptide receptors, neuroanatomy, physiology, behaviour, neurotrophic factors, preclinical drug evaluation, clinical studies, and clinical trials.