{"title":"Optical Investigation of DNA Behaviour in Flow using Confocal Detection Incorporated with Fluorescence Correlation Spectroscopy (FCS).","authors":"Fatma Doğan Güzel, Hamed Ghorbanpoor","doi":"10.1007/s10895-025-04263-5","DOIUrl":null,"url":null,"abstract":"<p><p>Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02-0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04263-5","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
Investigation of the behavior of biopolymers in flow such as Deoxyribonucleic acid (DNA), is a critical challenge in engineering, polymer, and life sciences. In this study, we studied the rheological properties of DNA and flow characteristics in real-time. The velocity measurement was carried out using confocal detection incorporated with Fluorescence Correlation Spectroscopy (FCS). Optical experiments provided an understanding of the diffusion- and flow-dominated regimes for molecules treated in microfluidic channels and lab-on-chip devices in general. We found that the flow-dominated regime starts at a flow rate of 0.3 µl/min and the transitional regime falls into 0.02-0.3 µl/min flow rates. There are a few examples for the detection of DNA and different fragments in flow as such. It is therefore believed to provide valuable insights into the subject of flow dynamics of DNA.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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